{"product_id":"pgbkt7-dna-bd-vector-630443","title":"pGBKT7 DNA-BD Vector","description":"\u003cp\u003epGBKT7 is a yeast expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the medium ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription\/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eActing as a bridge, by interacting with two proteins that do not directly interact with each other\u003c\/li\u003e\n\u003cli\u003eStabilizing a weak interaction between the two proteins\u003c\/li\u003e\n\u003cli\u003eModifying or inhibiting one or both of the proteins (Osbourne et al 1995)\u003c\/li\u003e\n\u003cli\u003eInhibiting the two-hybrid interaction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eYeast two-hybrid screening\u003cbr\u003eYeast three-hybrid screening\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/630443\/630443.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/630442\/630442-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGADT7%20AD%20Vector%20Information.pdf\"\u003epGADT7 AD Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGADT7%20AD.gb\"\u003epGADT7 AD Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45986019082319,"sku":"630443","price":3417.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/630443-pGBKT7-DNA-BD-Vector-product-600.jpg?v=1782801970","url":"https:\/\/www.danyel.co.il\/products\/pgbkt7-dna-bd-vector-630443","provider":"danyel biotech","version":"1.0","type":"link"}