{"product_id":"pet-express-purify-kit-histalon-631430","title":"pET Express \u0026 Purify Kit—HisTALON™","description":"\u003cp\u003eThe pET Express \u0026amp; Purify Kit-HisTALON allows you to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system includes IPTG-inducible, pET-based bacterial expression vectors that contain a T7\/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins. These proteins can be made exceptionally pure with the high-affinity HisTALON Gravity Columns included in the kit. The kit also includes a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNo copurification of unwanted host proteins\u003c\/li\u003e\n\u003cli\u003eNo SlyD contamination (histidine-rich protein present in E. coli)\u003c\/li\u003e\n\u003cli\u003eLowest metal ion leakage\u003c\/li\u003e\n\u003cli\u003eAvailable in various formats as bulk resin, cartridges, and gravity columns\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0438\/ST0438-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/His60%20Ni%20Superflow%20Resin%20and%20Gravity%20Columns%20User%20Manual\/His60%20Ni%20Superflow%20Resin%20and%20Gravity%20Columns%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631428-PA134460.pdf\"\u003epET Express \u0026amp; Purify Kit--His60 (In-Fusion Ready) Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pET6xHN-C_In-Fusion_Ready.gb\"\u003epET6xHN-C (In-Fusion Ready) vector sequence file in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pET6xHN-N_In-Fusion_Ready.gb\"\u003epET6xHN-N (In-Fusion Ready) vector sequence file in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631429-PA134461.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631430-PA134462.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631431-PA134463.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 Purifications","offer_id":45986026684495,"sku":"631430","price":5870.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/631430-pET-Express-and-Purify-Kit-HisTALON-product-600.jpg?v=1785957499","url":"https:\/\/www.danyel.co.il\/products\/pet-express-purify-kit-histalon-631430","provider":"danyel biotech","version":"1.0","type":"link"}