{"product_id":"pef1alpha-mcherry-n1-vector-631969","title":"pEF1alpha-mCherry-N1 Vector","description":"\u003cp\u003epEF1α-mCherry-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the Nterminus of the red fluorescent protein mCherry, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express mCherry in mammalian cells.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBright red fluorescent protein\u003c\/li\u003e\n\u003cli\u003eLentiviral (pLVX-) and retroviral (pRetroQ) formats are available\u003c\/li\u003e\n\u003cli\u003eRapid maturation (t0.5=15 min)\u003c\/li\u003e\n\u003cli\u003eMonomeric structure\u003c\/li\u003e\n\u003cli\u003eExcitation maximum: 587 nm\nEmission maximum: 610 nm\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCreate an in-frame fusion to track your protein of interest (-N and -C vectors)\u003cbr\u003eMonitor transfection\u003cbr\u003ePromoter-reporter studies\u003cbr\u003eQuantitative imaging techniques\u003cbr\u003eCoexpress your protein and another protein of interest using an IRES\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/631969\/631969.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/631969\/631969-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pEF1alpha-mCherry-N1%20Vector%20Information.pdf\"\u003epEF1alpha-mCherry-N1 Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pEF1a-mCherry-N1.gb\"\u003epEF1a-mCherry-N1  vector sequence in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45986030911567,"sku":"631969","price":3938.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/631969-pEF1alpha-mCherry-N1-Vector-product-600.jpg?v=1782805580","url":"https:\/\/www.danyel.co.il\/products\/pef1alpha-mcherry-n1-vector-631969","provider":"danyel biotech","version":"1.0","type":"link"}