{"product_id":"pd-minitrap-desalting-columns-with-sephadex-g-25-resin-28918007","title":"PD MiniTrap™ desalting columns with Sephadex G-25 resin","description":"\u003cp\u003ePD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFlexible protocols with the ability to use gravity flow or centrifugation.\u003c\/li\u003e\n\u003cli\u003eConvenient desalting and buffer exchange of sample volumes of 0.1 mL to 0.5 mL for gravity and 0.2 mL to 0.5 mL with centrifugation.\u003c\/li\u003e\n\u003cli\u003eReplaces dialysis for desalting protein, which saves time and reduces labor.\u003c\/li\u003e\n\u003cli\u003eFilled with Sephadex G-25 Medium resin.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eColumn for desalting and buffer exchange of proteins\u003c\/p\u003e\u003cp\u003ePD MiniTrap G-25 is designed for convenient desalting of biological samples. For increased flexibility, the protein desalting column can be used with either gravity or centrifugation.\u003c\/p\u003e\u003cp\u003eWhen using the gravity protocol, clean-up of the sample is simple. One or several columns can be connected in parallel, and a purification system is not needed. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge.The eluted sample will have minimal dilution.\u003c\/p\u003e\u003cp\u003eFour adapters are included in each product pack. The adapters simplify centrifugation of these protein desalting columns.\u003c\/p\u003e\u003cp\u003eThe exclusion limit is approximately M\u003csub\u003er\u003c\/sub\u003e 5000. This ensures group separation of proteins\/peptides larger than M\u003csub\u003er\u003c\/sub\u003e 5000 from smaller molecules.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003ePD MiniTrap G-25 are used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eBuffer exchange during \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4192493\/\"\u003ein vitro assembly of catalase\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRemoval of DTT during study of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4175335\/\"\u003ethiosulfate transfer in Metallosphaera cuprina\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eImidazole removal during study of disparate ligand \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4408927\/\"\u003ebinding sites in the human P2Y1 receptor\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eOther Cytiva products for buffer exchange or desalting\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume\u003c\/li\u003e\n\u003cli\u003ePD MidiTrap G-25 gravity column for sample volumes up to 1 mL\u003c\/li\u003e\n\u003cli\u003ePD-10 Desalting gravity column for sample volumes up to 2.5 mL\u003c\/li\u003e\n\u003cli\u003eHiTrap Desalting columns for sample volumes up to 1.5 mL. For use with a syringe, a peristaltic pump, or a chromatography system\u003c\/li\u003e\n\u003cli\u003eHiPrep Desalting column for sample volumes up to 15 mL. For use with a laboratory pump or a chromatography system\u003c\/li\u003e\n\u003cli\u003ePD MultiTrap G-25 for high-throughput desalting and buffer exchange of multiple samples\u003c\/li\u003e\n\u003cli\u003eThis resin is available as bulk resin\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14230-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28922529.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50 columns","offer_id":45982446682191,"sku":"28918007","price":1994.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8043-original-png_73c2a568-193f-42ba-a0e7-2cb213be3fbb.png?v=1785960274","url":"https:\/\/www.danyel.co.il\/products\/pd-minitrap-desalting-columns-with-sephadex-g-25-resin-28918007","provider":"danyel biotech","version":"1.0","type":"link"}