{"product_id":"pd-10-desalting-columns-packed-with-sephadex-g-25-resin-17085101","title":"PD-10 desalting columns packed with Sephadex™ G-25 resin","description":"\u003cp\u003ePD-10 Desalting Columns contain Sephadex™ G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFlexible run protocols – perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)\u003c\/li\u003e\n\u003cli\u003eFlexible throughput – desalt one sample or multiple samples in parallel\u003c\/li\u003e\n\u003cli\u003eGood recovery – in the range of 70% to \u0026gt; 95%\u003c\/li\u003e\n\u003cli\u003eHigh desalting capacity – remove \u0026gt; 98% salt with gravity; \u0026gt; 90% with centrifugation\u003c\/li\u003e\n\u003cli\u003eMinimal dilution – typically none when used as spin columns; 1.4× when used with gravity flow\u003c\/li\u003e\n\u003cli\u003eTime savings compared to dialysis – inexpensive and convenient alternative to lengthy and troublesome dialysis procedures\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhat's included in the PD-10 column package\u003c\/p\u003e\n\n\u003cp\u003eEach product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.\u003c\/p\u003e\n\n\u003cp\u003eVersatile for use with centrifugation or gravity flow\u003c\/p\u003e\n\n\u003cp\u003eThe product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.\u003c\/p\u003e\n\n\u003cp\u003eSuccessful use of these prepacked desalting columns\u003c\/p\u003e\n\n\u003cp\u003eThe following publications demonstrate the utility of PD-10 columns:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24196967\/\" target=\"_blank\"\u003eDesalting of membrane proteins\u003c\/a\u003e after anion exchange chromatography\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3911115\/\" target=\"_blank\"\u003eDesalting of variants\u003c\/a\u003e of an essential reductase involved in fatty acid biosynthesis\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4128096\/\" target=\"_blank\"\u003eBuffer exchange of an affinity-tagged\u003c\/a\u003e acyl carrier protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003eAccessories for PD-10 Desalting Columns\u003c\/p\u003e\n\n\u003cp\u003eA \u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/shop\/chromatography\/tools-and-accessories\/column-accessories\/buffer-reservoir-p-00403\" target=\"_blank\"\u003ebuffer reservoir\u003c\/a\u003e can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.\u003c\/p\u003e\n\n\u003cp\u003eOther Sephadex™ G-25 columns from Cytiva\u003c\/p\u003e\n\n\u003cp\u003eCytiva's solutions for desalting include a wide range of \u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/shop\/chromatography\/prepacked-columns\"\u003ecolumns containing Sephadex™ G-25 resin\u003c\/a\u003e. Options are available to accommodate sample volumes from 100 µL to 15 mL and use with manual methods or a chromatography system.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14230-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/52130800.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"30 columns","offer_id":45982338744399,"sku":"17085101","price":1485.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8047-original-png_afa6a6c2-f038-450b-b2b1-ad2fd691533f.png?v=1785960273","url":"https:\/\/www.danyel.co.il\/products\/pd-10-desalting-columns-packed-with-sephadex-g-25-resin-17085101","provider":"danyel biotech","version":"1.0","type":"link"}