{"product_id":"pcold-ii-dna-3362","title":"pCold™ II DNA","description":"\u003cp\u003eTakara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold II DNA contains a translation enhancing element (TEE) and his-tag sequence. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3362\/3362.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999847503,"sku":"3362","price":2974.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3362-pCold-II-DNA-product-600_50df317c-8c01-445b-aa45-84c7716de03d.jpg?v=1782801111","url":"https:\/\/www.danyel.co.il\/products\/pcold-ii-dna-3362","provider":"danyel biotech","version":"1.0","type":"link"}