{"product_id":"pbmc-24-spin-medium-density-gradient-medium-pluriselect","title":"PBMC 24+ Spin Medium, Density Gradient Medium","description":"\u003cp\u003ePBMC 24+ Spin Density Gradient Medium is a sterile, ready-to-use solution developed for the isolation of cells from older sample material. If the samples are already older than 6 hours, the yields and purities with conventional separation media for the enrichment of PBMC are considerably poorer. In addition, the cells are sometimes unsuitable for downstream applications. With PBMC 24+ Spin Medium, the time window between sample collection and cell enrichment is extended.\u003c\/p\u003e\u003ch2\u003eFeatures\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccess to older sample material\u003c\/strong\u003e – PBMC 24+ Spin Medium enables the enrichment of cells from sample material that is already older than 12 hours.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady to use\u003c\/strong\u003e – pluriSelect separation media are ready to use and can be used directly for cell separation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible\u003c\/strong\u003e – The enriched cells can be used for downstream applications such as MACS, EasySep, RosetteSep or MojoSort.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCombinable\u003c\/strong\u003e – PBMC 24+ Spin Medium can be combined with Leuko 24+ Spin Medium to form a double gradient for the simultaneous enrichment of two cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSeparation Scheme\u003c\/h2\u003e\u003cp\u003eFirst, the sample material is carefully layered onto the PBMC 24+ spin medium. After the density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The layer of mononuclear cells is then transferred to a new tube and the enriched cells are washed.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBEFORE\u003c\/strong\u003e\u003cbr\u003ePreparation of PBMC with PBMC 24+ Spin Medium. The sample material (1) is carefully placed on the PBMC 24+ Spin Medium (2).\u003c\/p\u003e\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-centrifugation-platelet-spin-v3.webp\" alt=\"PBMC 24+ Spin Medium, Density Gradient Medium separation scheme\" style=\"max-width:100%\"\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAFTER\u003c\/strong\u003e\u003cbr\u003eLayers after centrifugation. The PBMC (2) are located as a white layer on the PBMC 24+ spin medium (3). Cells with a higher density, such as erythrocytes, granulocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).\u003c\/p\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSterility\u003c\/th\u003e\n\u003ctd\u003esterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation platform\u003c\/th\u003e\n\u003ctd\u003enon-magnetic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation method\u003c\/th\u003e\n\u003ctd\u003enegative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct type\u003c\/th\u003e\n\u003ctd\u003eCell separation reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReagent type\u003c\/th\u003e\n\u003ctd\u003eDensity gradient medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eDensity gradient centrifugation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTerms of delivery\u003c\/th\u003e\n\u003ctd\u003eambient temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage condition\u003c\/th\u003e\n\u003ctd\u003eroom temperature, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRegulatory statement\u003c\/th\u003e\n\u003ctd\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget cells\u003c\/th\u003e\n\u003ctd\u003eMononuclear cells (lymphocytes, monocytes)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSeparation density cut-off\u003c\/th\u003e\n\u003ctd\u003e1,072 g\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample material\u003c\/th\u003e\n\u003ctd\u003ehuman whole blood, Buffy Coat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible with\u003c\/th\u003e\n\u003ctd\u003epluriMate, SepMate, LeucoSep\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCan be used with\u003c\/th\u003e\n\u003ctd\u003epluriSpin, MACS, EasySep, RosetteSep, MojoSort\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003cth\u003ePacking\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00093-10\u003c\/td\u003e\n\u003ctd\u003e100 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00093-11\u003c\/td\u003e\n\u003ctd\u003e250 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00093-12\u003c\/td\u003e\n\u003ctd\u003e500 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"pluriSelect","offers":[{"title":"100 ml","offer_id":68092788441167,"sku":"60-00093-10","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"250 ml","offer_id":68092788473935,"sku":"60-00093-11","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"500 ml","offer_id":68092788506703,"sku":"60-00093-12","price":316.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-product-291.jpg?v=1794313283","url":"https:\/\/www.danyel.co.il\/products\/pbmc-24-spin-medium-density-gradient-medium-pluriselect","provider":"danyel biotech","version":"1.0","type":"link"}