{"product_id":"one-step-primescript-iii-rt-qpcr-mix-with-ung-rr601b","title":"One Step PrimeScript™ III RT-qPCR Mix, with UNG","description":"\u003cp\u003eOne Step PrimeScript III RT-qPCR Mix, with UNG is a dedicated reagent for one-step, real-time, probe-based RT-qPCR (using the 5ʼ nuclease method). This 2X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. Because the premix contains Uracil N-Glycosylase (UNG), which degrades amplification products from the previous reaction, it can prevent carryover contamination even when a specific target is repeatedly tested for the purpose of inspection, etc. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix, with UNG is also highly resistant to a wide variety of inhibitory substances such as heparin (blood) and humic acid (soil), allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc. Cat. # RR601B contains five of Cat. # RR601A. Please refer to Cat. # RR601A for complete product documentation and resources.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFast, easy one-step RT-qPCR in a single tube using probe-based detection\u003c\/li\u003e\n\u003cli\u003eEfficient, specific cDNA synthesis and amplification, even from complex RNA templates\u003c\/li\u003e\n\u003cli\u003eSuitable for a wide variety of samples from different sources, including crude RNA extracts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGene expression\u003cbr\u003eRNA virus detection\u003cbr\u003eMultiplex real-time PCR\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD7079\/SD7079-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/PrimePath%20Direct%20Saliva%20SARS\/PrimePath%20Direct%20Saliva%20SARS-CoV-2%20Detection%20Kit%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1,000 Rxns","offer_id":45986318843983,"sku":"RR601B","price":9626.0,"currency_code":"ILS","in_stock":true}],"url":"https:\/\/www.danyel.co.il\/products\/one-step-primescript-iii-rt-qpcr-mix-with-ung-rr601b","provider":"danyel biotech","version":"1.0","type":"link"}