{"product_id":"nxgen-t7-rna-polymerase-30223-1","title":"NxGen T7 RNA Polymerase","description":"\u003cp\u003eT7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eReliable transcription.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37 °C.\u003c\/p\u003e\u003cp\u003eSource: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.\u003c\/p\u003e\u003cp\u003eUnit Concentration\t50,000 U\/mL\u003cbr\u003ePurity (SDS-PAGE)\t\u0026gt;99%\u003cbr\u003eSS Exonuclease\t500 U \u0026lt;0.1% released\u003cbr\u003eDS Exonuclease\t500 U \u0026lt;0.1% released\u003cbr\u003eEndonuclease\t500 U \u0026lt;10% converted\u003cbr\u003eRNase Contamination\t500 U = none detected\u003cbr\u003eE. coli 16S rDNA Contamination\t500 U \u0026lt;10 copies\u003cbr\u003eStorage\t-20 °C\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eSupplied at 50,000 U\/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30223-1_SDS-30223-NxGen-T7-RNA-Polymerase\"\u003eSDS 30223 NxGen T7 RNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30223-1_T7-RNAP\"\u003eT7 RNAP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_30223-1_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986431205455,"sku":"30223-1","price":1015.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-T7-RNA-Polymerase_27fb9e79-95df-4abb-90d3-e47cada8fbdd.jpg?v=1785956107","url":"https:\/\/www.danyel.co.il\/products\/nxgen-t7-rna-polymerase-30223-1","provider":"danyel biotech","version":"1.0","type":"link"}