{"product_id":"nxgen-phi29-dna-polymerase-pcramp-011","title":"NxGen phi29 DNA Polymerase","description":"\u003cp\u003eNxGen Phi29 DNA Polymerase is a highly processive, high-fidelity DNA polymerase that is derived from bacteriophage phi29.\u003c\/p\u003e\u003cp\u003eIt is an isothermal DNA polymerase with high strand displacement activity and a 3′→ 5′ proofreading exonuclease function that can amplify DNA with high specificity and accuracy, even from limited or degraded samples.\u003c\/p\u003e\u003cp\u003eIdeal applications:\u003c\/p\u003e\u003cp\u003eRolling Circle Amplification (RCA)\u003c\/p\u003e\u003cp\u003eWhole Genome Amplification (WGA)\u003c\/p\u003e\u003cp\u003eStrand Displacement Activity\u003c\/p\u003e\u003cp\u003eThe high processivity of the enzyme allows for efficient amplification of long DNA fragments up to 70,000 base insertions per binding event, while the high-fidelity ensures that the amplified DNA is of high quality and accuracy.\u003c\/p\u003e\u003cp\u003eFigure 1. Whole genome amplification as outlined in Protocol 2 of this manual. Five different lots of NxGen phi29 DNA Polymerase were used and duplicate NTC and (+) human genomic amplification reactions were set up. Amplification reactions were incubated for 16 hours at 30 °C and products were analysed in a 0.7% agarose gel and stained with ethidium bromide to visualise amplified DNA. The red arrows indicate NTC reactions with detectable background (input template independent) amplification.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePowerful strand displacement activity, making it ideal for challenging samples or damaged DNA\u003c\/li\u003e\n\u003cli\u003eHighly processive, allowing for amplification of up to 70,000 base insertions per binding event\u003c\/li\u003e\n\u003cli\u003eHigh-fidelity 3'-\u0026gt;5' proofreading exonuclease function for accurate DNA replication with low error rates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.\u003c\/p\u003e\u003cp\u003eSource: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe NxGen Phi29 DNA Polymerase sources its enzyme from a recombinant E. coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.\u003c\/p\u003e\u003cp\u003e1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30 °C.\u003c\/p\u003e\u003cp\u003eThe enzyme storage buffer consists of 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25 °C and the 10X phi29 DNA Polymerase Buffer is composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-011_30221-NxGen-phi29-DNA-Polymerase\"\u003e30221 NxGen phi29 DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-011_phi29\"\u003ephi29\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-011_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 U\/µL, 2,000 U","offer_id":45986430845007,"sku":"30221-1","price":1149.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/µL, 10,000 U","offer_id":45986430877775,"sku":"30221-2","price":4466.0,"currency_code":"ILS","in_stock":true},{"title":"50,000 U at 10,000 U\/mL","offer_id":45986430910543,"sku":"30221-3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mL at 10,000 U\/mL, 50,000 U","offer_id":45986430943311,"sku":"F83900-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-phi29-DNA-Polymerase_2bd500de-592d-4cb3-8749-e6343fa17169.jpg?v=1785956103","url":"https:\/\/www.danyel.co.il\/products\/nxgen-phi29-dna-polymerase-pcramp-011","provider":"danyel biotech","version":"1.0","type":"link"}