{"product_id":"nxgen-m-mulv-reverse-transcriptase-30222-1","title":"NxGen M-MuLV Reverse Transcriptase","description":"\u003cp\u003eM-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37 °C using poly r(A)\/oligo (dT) as a substrate.\u003c\/p\u003e\u003cp\u003eSource: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.\u003c\/p\u003e\u003cp\u003eUnit Concentration\t200,000 U\/mL\u003cbr\u003ePurity (SDS-PAGE)\t\u0026gt;99%\u003cbr\u003eSS Exonuclease\t2,000 U \u0026lt;5.0% released\u003cbr\u003eDS Exonuclease\t2,000 U \u0026lt;0.5% released\u003cbr\u003eEndonuclease\t2,000 U \u0026lt;10% converted\u003cbr\u003eE. coli 16S rDNA Contamination\t2,000 U \u0026lt;10 copies\u003cbr\u003eStorage\t-20 °C\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eSupplied at 200,000 U\/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 °C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 °C. 250,000 U is supplied as 5 × 50,000 U.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30222-1_SDS-30222-NxGen-M-MuLV-Reverse-Transcriptase\"\u003eSDS 30222 NxGen M MuLV Reverse Transcriptase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30222-1_MA115-M-MuLV\"\u003eMA115 M MuLV\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_30222-1_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986431107151,"sku":"30222-1","price":1102.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-M-MuLV-Reverse-Transcriptase_76af2cc0-8939-42c5-b17a-a067ba9a1869.jpg?v=1785956106","url":"https:\/\/www.danyel.co.il\/products\/nxgen-m-mulv-reverse-transcriptase-30222-1","provider":"danyel biotech","version":"1.0","type":"link"}