{"product_id":"ni-sepharose-excel-histidine-tagged-protein-purification-resin-17371203","title":"Ni Sepharose™ excel histidine-tagged protein purification resin","description":"\u003cp\u003eNi Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVersatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.\u003c\/li\u003e\n\u003cli\u003eDirect sample load – of eukaryotic cell culture supernatants, eliminating the need for  pretreatment of samples that usually cause nickel stripping.\u003c\/li\u003e\n\u003cli\u003eRetained binding capacity of up to 10 mg\/mL resin.\u003c\/li\u003e\n\u003cli\u003eSimplified sample handling – fewer steps needed compared with conventional workflow.\u003c\/li\u003e\n\u003cli\u003eFlexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEDTA-resistant resin for IMAC chromatography\u003c\/p\u003e\u003cp\u003eNi Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.\u003c\/p\u003e\u003cp\u003eBecause of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.\u003c\/p\u003e\u003cp\u003eHis tag purification of large sample volumes\u003c\/p\u003e\u003cp\u003eThe flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eScientists use Ni Sepharose excel to purify his-tagged recombinant proteins from eukaryotic and prokaryotic samples, as cited in these publications:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHuman transferrin receptor 1 secreted  \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4051223\/\"\u003efrom BHK cells\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eProprotein convertase subtilisin\/kexin type 9 \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25778403\/\"\u003efrom HEK293 supernatants\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMembrane protein insertase EcYidC \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4252904\/\"\u003efrom \u003ci\u003eE. coli\u003c\/i\u003e for crystallization\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eHelicobacter pylori adhesin BabA \u003ca href=\"https:\/\/www.nottingham.ac.uk\/research\/groups\/allergy-and-infectiousdiseases\/news\/protein-expression.aspx\"\u003efrom periplasmic extracts\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eConvenient prepacked columns\u003c\/p\u003e\u003cp\u003eNi Sepharose excel resin is also available in the following formats:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.\u003c\/li\u003e\n\u003cli\u003eHis MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16213-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29013867.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"25 mL","offer_id":45982407655503,"sku":"17371201","price":2533.0,"currency_code":"ILS","in_stock":true},{"title":"100 mL","offer_id":45982407688271,"sku":"17371202","price":9718.0,"currency_code":"ILS","in_stock":true},{"title":"500 mL","offer_id":45982407721039,"sku":"17371203","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-3788-original-png_484dcce4-e549-458e-b084-309509a70da5.png?v=1785960772","url":"https:\/\/www.danyel.co.il\/products\/ni-sepharose-excel-histidine-tagged-protein-purification-resin-17371203","provider":"danyel biotech","version":"1.0","type":"link"}