{"product_id":"ni-sepharose-6-fast-flow-histidine-tagged-protein-purification-resin-17531804","title":"Ni Sepharose™ 6 Fast Flow histidine-tagged protein purification resin","description":"\u003cp\u003eNi Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIn process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.\u003c\/li\u003e\n\u003cli\u003eIn research scale applications: This resin binds 40 mg his-tag protein\/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.\u003c\/li\u003e\n\u003cli\u003eMeets industrial demands with security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003cli\u003eAvailable as bulk resin and as prepacked columns for use with manual methods or a chromatography system.\u003c\/li\u003e\n\u003cli\u003eExcellent flow properties provide great performance even at high flow rates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHigh binding capacity resin with excellent flow properties\u003c\/p\u003e\u003cp\u003eNi Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.\u003c\/p\u003e\u003cp\u003eThe high dynamic binding capacity combined with the ability to withstand high flow rates gives an overall advantage of being able to increase flow rate and reduce the total time for protein purification considerably with a minimal loss in recovery. Therefore, this immobilized metal affinity chromatography (IMAC) purification resin is a good choice for scaling up.\u003c\/p\u003e\u003cp\u003eSuccessful use in research-scale applications\u003c\/p\u003e\u003cp\u003eLife science professionals use Ni Sepharose 6 Fast Flow for his-tag protein purification:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/www.biocompare.com\/Product-Reviews\/40379-GE-Healthcare-s-Ni-Sepharose-6-Fast-Flow\/\"\u003eCustomer feedback\u003c\/a\u003e on use in batch purification\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26964310\/\"\u003eIMAC chromatography of human β2-microglobulin\u003c\/a\u003e in \u003ci\u003eE. coli\u003c\/i\u003e to 95% purity\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19395325\/\"\u003eScale-up purification\u003c\/a\u003e of a viral capsid protein from a prepacked column\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatibile empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva offers a variety of empty columns for packing your own columns. Ni Sepharose 6 Fast Flow is compatible for use with empty PD gravity flow, HiScale, Tricorn, and XK columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications, Cytiva recommends AxiChrom columns. Ni Sepharose 6 Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003ePrepacked formats – research scale\u003c\/p\u003e\u003cp\u003eNi Sepharose 6 Fast Flow resin is also available in the following prepacked formats:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHisGraviTrap gravity flow column: for simple purification without the need for equipment\u003c\/li\u003e\n\u003cli\u003eHis MultiTrap FF 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment\u003c\/li\u003e\n\u003cli\u003eHisTrap FF crude: HiTrap column format for convenient use with a syringe, a peristaltic pump or a chromatography system.\u003c\/li\u003e\n\u003cli\u003eHiScreen Ni FF column for method and process development\u003c\/li\u003e\n\u003cli\u003eHisPrep FF column for convenient scale-up\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePrepacked formats – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadytoProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.\u003c\/p\u003e\u003cp\u003eOther IMAC chromatography resins and columns\u003c\/p\u003e\u003cp\u003eLife Sciences solutions from Cytiva include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, and loading of unclarified \u003ci\u003eE. coli\u003c\/i\u003e lysates are available.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13253-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13803-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 L","offer_id":45982039474255,"sku":"17531804","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mL","offer_id":45982039507023,"sku":"17531806","price":499.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL","offer_id":45982039539791,"sku":"17531801","price":2023.0,"currency_code":"ILS","in_stock":true},{"title":"100 mL","offer_id":45982039572559,"sku":"17531802","price":5306.0,"currency_code":"ILS","in_stock":true},{"title":"500 mL","offer_id":45982039605327,"sku":"17531803","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Standard","offer_id":45982039638095,"sku":"17531805","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-5613-original-png_3f3b7372-c104-415a-975f-b9ed57155a61.png?v=1785957548","url":"https:\/\/www.danyel.co.il\/products\/ni-sepharose-6-fast-flow-histidine-tagged-protein-purification-resin-17531804","provider":"danyel biotech","version":"1.0","type":"link"}