{"product_id":"neuraminidase-activity-fluorometric-assay-tbs2109","title":"Neuraminidase Activity Fluorometric Assay","description":"\u003cp\u003eNeuraminidase (NA, EC 3.2.1.18) is a type of glycoside hydrolase enzyme present in many organisms. It cleaves sialic acid residues from glycoproteins and glycolipids. This action is essential for many biological processes. It plays a critical role in viral replication and cellular processes, particularly in influenza virus infection. Therefore, viruses are often classified by neuraminidase subtypes, such as N1, N2, etc. Neuraminidase is related to neurodegenerative disease, such as Alzheimer’s disease, and Parkinson’s disease.\u003c\/p\u003e\n\u003cp\u003eThe Neuraminidase Activity Fluorometric Assay provides a simple, accurate, and sensitive method for monitoring neuraminidase activity in biological samples. In this assay, neuraminidase cleaves a non-fluorescent substrate to release a fluorescent product, which can be measured at an excitation wavelength of 365 nm and an emission wavelength of 445 nm.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNeuraminidase activity in biological samples.\u003c\/li\u003e\n\u003cli\u003eInfuenza virus antigen titration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimplicity: \u003c\/strong\u003eThe assay directly detects enzymatic activity without complex enzyme coupling steps.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e Even low levels of neuraminidase activity can be detected due to the high fluorescence intensity.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKit Contents for 100 Assays\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 80%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e\u003cstrong\u003ePart Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003eSubstrate (50X)\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e120 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003eStandard (1 mM)\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003ePositive control (12X)\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003eStop Reagent\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"150\"\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd width=\"91\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage and Shipment Conditions\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is shipped on ice and should be stored at -20°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard curve:\u003c\/strong\u003e This standard curve in Fig 2 is provided as a reference demonstration. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-35803 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2109_01-1.png\" alt=\"\" width=\"570\" height=\"373\" data-lazy-sizes=\"auto, (max-width: 570px) 100vw, 570px\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.3 displays the NA enzyme activity analyzed at different time points of incubation at 37℃.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-35802 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2109_02-1.png\" alt=\"\" width=\"577\" height=\"476\" data-lazy-sizes=\"auto, (max-width: 577px) 100vw, 577px\" data-was-processed=\"true\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2026\/01\/TBS2109_Neuraminidase-Fluorometric-assay-datasheet-V02_2025.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2109-Neuraminidase-Activity-Fluorometric_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986453061711,"sku":"TBS2109","price":1732.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2109.png?v=1782811273","url":"https:\/\/www.danyel.co.il\/products\/neuraminidase-activity-fluorometric-assay-tbs2109","provider":"danyel biotech","version":"1.0","type":"link"}