{"product_id":"mutarotase-enzyme-activity-tbp0081","title":"Mutarotase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Ammonium Sulfate\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Soluble in distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Stable when stored at 4°C. Do not freeze\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 5,000 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 10 mg\/ml\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThat amount of enzyme which will increase the spontaneous mutarotation of one micromole of a-D-Glucose to §-D-Glucose per minute at pH 7.4 and 25°C. One rotation unit is equal to 0.04 U when measured with Glucose dehydrogenase.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe rate at which the spontaneous mutarotation of a-D-Glucose is increased at 25°C at pH 7.4 in the presence of mutarotase as measured by a precision polarimeter.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eMutarotase (EC 5.1.3.3) catalyzes the mutarotation of a-D-Glucose to ß-D-Glucose as shown in the following reaction:\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eMutarotase, which catalyzes the mutarotation of certain sugars was discovered by accident in 1949. Keston reported the occurrence of an enzyme of similar characteristics in animal tissues such as rat liver and kidneys of porcine, rabbit, chicken, rat, bovine and lamb. Later, Keston reported partial purification by adsorption with phosphate gel and chloroform treatment. He has also considered the kinetics and distribution of mutarotases of various animals and their possible relation to sugar transport. A controversial question concerning the postulated inhibition of kidney mutarotase by its own substrate, glucose, has been resolved.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e5.0 mM sodium EDTA, pH 7.4.\u003c\/li\u003e\n\u003cli\u003eEnzyme Solution. Freshly prepared (just before use) enzyme solution in reagent (1) containing approximately 200 units per ml.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eProcedure\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDetermination of Spontaneous Mutarotation:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eAt time zero dissolve 100 mg (accurately weighed) a-D-Glucose in 10.0 ml reagent (1) and rapidly transfer to polarimeter cell thermostated at 25°C. Record the rotation at one minute intervals for the first 10 minutes. After 10 minutes record the rotation at 5 minute intervals. After 30 minutes record the rotation at 15 minute intervals. Continue until rotation is constant. This should occur in about 4 hours and should be approximately +53°C.\u003c\/p\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eGraph rotation vs. time. Extrapolate to zero to obtain the initial rotation. The initial rotation corresponds to an a-D- Glucose concentration of 555 micromole per reaction mixture.\u003c\/li\u003e\n\u003cli\u003eBlank Rotation Graph: Graph rotation vs. time in 1.0 minute intervals. Determine change in rotation for 5.0 minutes.\u003c\/li\u003e\n\u003cli\u003eStandard Curve: Graph rotation vs. micromole a-D-Glucose using data from spontaneous mutarotation. There are two points. Initial is 555 micromole and final rotation is 195 micromole.\u003c\/li\u003e\n\u003cli\u003eDetermination of Test Rotation:\n\u003col\u003e\n\u003cli\u003eAt time zero add 0.10 ml enzyme solution to 9.9 ml reagent (1) and rapidly dissolve exactly 100 mg a-D-Glucose in this mixture. Determine rotation at 30 second intervals for ten minutes.\u003c\/li\u003e\n\u003cli\u003eAt time zero add 0.05 ml enzyme solution to 9.95 ml reagent (1) and rapidly dissolve exactly 100 mg a-D-Glucose in this mixture. Determine rotation at 30 second intervals for ten minutes.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0081-1KU TBP0081-5KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0080-Lysozyme.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0081_Mutarotase-Dehydrogenase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 KU","offer_id":45986500345935,"sku":"TBP0081-1KU","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 KU","offer_id":45986500378703,"sku":"TBP0081-5KU","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0081.png?v=1782812668","url":"https:\/\/www.danyel.co.il\/products\/mutarotase-enzyme-activity-tbp0081","provider":"danyel biotech","version":"1.0","type":"link"}