{"product_id":"mouse-feeder-cell-quantification-kit-rr290","title":"Mouse Feeder Cell Quantification Kit","description":"\u003cp\u003eThe Mouse Feeder Cell Quantification Kit is designed to facilitate highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells using real-time PCR to analyze genomic DNA extracted from stem cell cultures (e.g., hESC, hiPSC). The kit includes primers for real-time PCR that were designed to detect a mouse mitochondrial (mt) DNA sequence, allowing detection of mouse feeder cells with high sensitivity. The copy number of cellular mtDNA is known to vary depending on cell type, cell line, and differentiation state, and there is a possibility of fluctuation betweenmouse feeder cell lots. The primer set for quantification of mouse mtDNA copy number included in this kit can be used for lot-to-lot correction of mouse feeder cells.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes real-time PCR to detect and quantify residual mouse feeder cells\u003c\/li\u003e\n\u003cli\u003eProvides mtDNA PCR primers to make mouse mtDNA sequence detection easy and sensitive\u003c\/li\u003e\n\u003cli\u003eIncludes nuclear DNA PCR primers for normalization to quantify mouse mtDNA copy number\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eAssess the level of mouse cell contamination in stem cell cultures grown using mouse feeder cells\u003cbr\u003eCompare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/RR290\/RR290.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD5043\/SD5043-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/RR290\/RR290_UM.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 Rxns","offer_id":45986315829327,"sku":"RR290","price":4487.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RR290-Mouse-Feeder-Cell-Quantification-Kit-product-600.jpg?v=1782808363","url":"https:\/\/www.danyel.co.il\/products\/mouse-feeder-cell-quantification-kit-rr290","provider":"danyel biotech","version":"1.0","type":"link"}