{"product_id":"mouse-c5a-elisa-kit-tbs3001-01","title":"Mouse C5a ELISA Kit","description":"\u003cp\u003eComplement component C5a is a multifunctional proinflammatory mediator produced by complement cascade activation and causes inflammatory responses and cytokine production in septic shock, allergy, trauma, ischemic heart disease, pain, neurodegenerative diseases, and several other autoimmune diseases. C5a is rapidly cleaved to the C5a-DesArg form by the endogenous serum carboxypeptidase enzyme.\u003c\/p\u003e\n\u003cp\u003eThe C5a ELISA Kit can be used for \u003ci\u003ein vitro\u003c\/i\u003e determination of mouse C5a in different tissue samples including skin, muscle, nervous system and other biological samples. The microplate is coated with murine C5a monoclonal antibody. C5a present in the sample or standard binds to the antibodies absorbed to the microwell. A biotin-conjugated anti-murine C5a antibody is then added and binds to murine C5a captured by the first antibody. After adding streptavidin-horseradish peroxidase (HRP) an antibody-antigen-antibody “sandwich” is produced. Finally, substrate solution reactive with HRP is added to the well, exhibiting a blue color in direct proportion to the amount of C5a in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450nm. The concentration of C5a in the samples is determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDirect Assays: C5a concentrations in different tissues.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity:\u003c\/strong\u003e The minimum detectable dose of mouse C5a is typically less than 7.8 pg\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e This assay recognizes recombinant and natural mouse C5a. No significant cross-reactivity or interference is observed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at 4℃ and standard at -20℃, respectively.\u003cbr\u003e\nShelf life: 12 months after receipt.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3001-01 TBS3001-02--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/03\/TBS3001-01_mouseC5a_V02_03052020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3001_Mouse-C5a_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1x96-well plate","offer_id":45986370519119,"sku":"TBS3001-01","price":1457.0,"currency_code":"ILS","in_stock":true},{"title":"2x96-well plate","offer_id":45986370551887,"sku":"TBS3001-02","price":2553.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3001.jpg?v=1782809665","url":"https:\/\/www.danyel.co.il\/products\/mouse-c5a-elisa-kit-tbs3001-01","provider":"danyel biotech","version":"1.0","type":"link"}