{"product_id":"mouse-c3a-elisa-kit-tbs3020-01","title":"Mouse C3a ELISA Kit","description":"\u003cp\u003eComplement component C3a anaphylatoxin is a multifunctional proinflammatory mediator produced by complement cascade activation and causes inflammatory responses and cytokine production in septic shock, allergy, trauma, ischemic heart disease, pain, neurodegenerative diseases, and several other autoimmune diseases. C3a is rapidly cleaved to the C3a-DesArg form by the endogenous serum carboxypeptidase enzyme.\u003cbr\u003e\nThe C3a ELISA Kit is for the \u003ci\u003ein vitro\u003c\/i\u003e determination of mouse C3a in different tissue samples including skin, muscle, nervous system and other biological samples. The microplate is coated with murine C3a monoclonal antibody. C3a present in the sample or standard binds to the antibodies absorbed by the microwells. A biotin-conjugated anti-murine C3a antibody is added and binds to murine C3a captured by the first antibody. Next, streptavidin-horseradish peroxidase (HRP) is added, producing an antibody-antigen-antibody “sandwich”. Finally, substrate solution reactive with HRP is added to the well and exhibits a blue color that is directly proportional to the amount of C3a in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450nm. The concentration of C3a in the samples is determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDirect Assays:\u003c\/strong\u003e C3a concentrations in different tissues.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity:\u003c\/strong\u003e The minimum detectable dose of mouse C3a is typically less than 7.8 pg\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e This assay recognizes recombinant and natural mouse C3a. No significant cross-reactivity or interference is observed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 388px;height: 288px\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecoated 96-well Plate\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e1x 100μ:\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent (10x concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Solution (20x Concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at 4℃ and standard at -20℃, respectively.\u003cbr\u003e\nShelf life: 12 months after receipt.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/03\/TBS3020-01_mouseC3a_V02_03052020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3020_Mouse-C3a_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1x96-well plate","offer_id":45986370715727,"sku":"TBS3020-01","price":1457.0,"currency_code":"ILS","in_stock":true},{"title":"2x96-well plate","offer_id":45986370748495,"sku":"TBS3020-02","price":2553.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3020.jpg?v=1782809669","url":"https:\/\/www.danyel.co.il\/products\/mouse-c3a-elisa-kit-tbs3020-01","provider":"danyel biotech","version":"1.0","type":"link"}