{"product_id":"mitochondrial-complex-iii-activity-colorimetric-assay-tbs2019","title":"Mitochondrial Complex III Activity Colorimetric Assay","description":"\u003cp\u003eMitochondria Complex III, or Ubiquinol-cytochrome c reductase (EC1.10.2.2), plays a crucial role in the oxidative phosphorylation process in mitochondria, which is essential for ATP production. Mutations of mitochondria Complex III were reported to be involved in many diseases’ pathologies, such as neuron degenerative diseases, cardiovascular diseases, cancer and diabetes. It catalyzes the reduction of cytochrome c by oxidation of coenzyme Q (CoQ) and the accompany pumping of 4 protons from the mitochondrial matrix to the intermembrane space, as shown in\u003cstrong\u003e Fig.1\u003c\/strong\u003e below.\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-33582 size-full lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/08\/TBS2019_01-1.png\" alt=\"\" width=\"600\" height=\"382\" data-lazy-sizes=\"auto, (max-width: 600px) 100vw, 600px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003eThe activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eComplex III Activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents for 100 Tests:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 60%\" border=\"1px solid line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"204\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cstrong\u003ePart Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"204\"\u003eComplex III Assay Buffer\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"204\"\u003eCytochrome c (2 mM)\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"204\"\u003eDTT (2x)\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e0.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"204\"\u003eComplex III Inhibitor Antimycin A\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e0.2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStorage conditions: Store the reagent at –20°C protected from light.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/08\/TBS2019-Complex-III-activity-assay-V04-2025.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/08\/TBS2019-Complex-III-Colorimetric_assay_kit_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986420392015,"sku":"TBS2019","price":2231.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2019-1.jpg?v=1782810749","url":"https:\/\/www.danyel.co.il\/products\/mitochondrial-complex-iii-activity-colorimetric-assay-tbs2019","provider":"danyel biotech","version":"1.0","type":"link"}