{"product_id":"microspin-columns-27356501","title":"MicroSpin Columns","description":"\u003cp\u003eDesigned for the rapid purification of DNA, in conjunction with Sephadex G-25 DNA Grade or Sephadex G-50 DNA Grade , for desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor rapid buffer exchange\/desalting of PCR products and other DNA samples in a volume of 10 to 100 µL using spin-column chromatography and Sephadex G-25 DNA Grade (not included)\u003c\/li\u003e\n\u003cli\u003eExcellent for rapid purification of newly synthesized oligonucleotides \u0026gt; 10-mers in 100 to 150 µL of deprotection solution using spin-column chromatography\u003c\/li\u003e\n\u003cli\u003eFlexible for your experiments, allowing differing amounts of Sephadex\u003c\/li\u003e\n\u003cli\u003eCan also be used for desalting\/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eBoth types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-25-dna-grade-p-00054\"\u003eSephadex G-25 DNA Grade\u003c\/a\u003e and \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-50-dna-grade-p-00113\"\u003eSephadex G-50 DNA Grade\u003c\/a\u003e are sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":45982268129359,"sku":"27356501","price":1028.0,"currency_code":"ILS","in_stock":true}],"url":"https:\/\/www.danyel.co.il\/products\/microspin-columns-27356501","provider":"danyel biotech","version":"1.0","type":"link"}