{"product_id":"mc1061-f-electrocompetent-cells-comcel-012","title":"MC1061 F-Electrocompetent Cells","description":"\u003cp\u003eMC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu\/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F- Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for antibody phage display or peptide phage display library creation\u003c\/li\u003e\n\u003cli\u003eThe highest efficiency TG1 competent cells available\u003c\/li\u003e\n\u003cli\u003eYour only source for electrocompetent SS320 and ER2738 cells\u003c\/li\u003e\n\u003cli\u003eAmber suppressor and non-amber suppressor lines for maximum flexibility\u003c\/li\u003e\n\u003cli\u003eHigh efficiency MC1061 F- cells for cloning or control reactions\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-012_60514-1-60514-2-MC1061-F-Electrocompetent-Cells\"\u003e60514 1 60514 2 MC1061 F Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-012_60514-MC1061-F-Electrocompetent-Cells\"\u003e60514 MC1061 F Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-012_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-012_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-012_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-012_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433663055,"sku":"60514-1","price":1219.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433695823,"sku":"60514-2","price":2097.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MC1061-F-Electrocompetent-Cells_2820ecd1-4d77-42b7-8e8e-9ebb14367809.jpg?v=1785956152","url":"https:\/\/www.danyel.co.il\/products\/mc1061-f-electrocompetent-cells-comcel-012","provider":"danyel biotech","version":"1.0","type":"link"}