{"product_id":"mate-plate-library-universal-arabidopsis-normalized-630487","title":"Mate \u0026 Plate™ Library - Universal Arabidopsis (Normalized)","description":"\u003cp\u003eThis yeast two-hybrid library was constructed from mRNA isolated from 11 Arabidopsis tissues, mixed in equal quantities and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low copy number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMART technology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions. The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBy far the easiest way to screen a library for protein-protein interactions\u003c\/li\u003e\n\u003cli\u003eScreen fewer colonies, detect more interactions\u003c\/li\u003e\n\u003cli\u003eUniversal libraries—broadest gene representation\u003c\/li\u003e\n\u003cli\u003eNo more searching for needles in a haystack\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eYeast two-hybrid library screening\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/630487\/630487-LOT1103583A.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0035\/ST0035-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Matchmaker%20Gold%20Yeast%20Two\/Matchmaker%20Gold%20Yeast%20Two-Hybrid%20System%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGADT7-RecAB%20Vector%20Information.pdf\"\u003epGADT7-RecAB Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGADT7-RecAB.gb\"\u003epGADT7-RecAB Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e630489\u003c\/td\u003e\n\u003ctd\u003eMatchmaker® Gold Yeast Two-Hybrid System\u003c\/td\u003e\n\u003ctd\u003eEach\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"5 x 1 mL","offer_id":45986020786255,"sku":"630487","price":9018.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/630487-Mate-and-Plate-Library-Universal-Arabidopsis-Normalized-product-600.jpg?v=1782801993","url":"https:\/\/www.danyel.co.il\/products\/mate-plate-library-universal-arabidopsis-normalized-630487","provider":"danyel biotech","version":"1.0","type":"link"}