{"product_id":"matchmaker-insert-check-pcr-mix-2-630497","title":"Matchmaker® Insert Check PCR Mix 2","description":"\u003cp\u003eMatchmaker Insert Check PCR Mix 2 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix and perform 30 cycles of PCR. Matchmaker Insert Check PCR Mix 2 is designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively) to amplify and screen cDNA inserts from prey\/library plasmids following yeast one- and two-hybrid screening. The Mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7- Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQuick, easy PCR amplification directly from yeast colonies\u003c\/li\u003e\n\u003cli\u003eComplete premix contains primers, dNTPs, and buffer—just add your cells and go!\u003c\/li\u003e\n\u003cli\u003ePrimers are also available separately\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eYeast two-hybrid screening\u003cbr\u003eYeast one-hybrid screening\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/630497\/630497.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S3553\/S3553-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 Rxns","offer_id":45986021245007,"sku":"630497","price":1122.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/630497-Matchmaker-Insert-Check-PCR-Mix-2-product-600.jpg?v=1782802010","url":"https:\/\/www.danyel.co.il\/products\/matchmaker-insert-check-pcr-mix-2-630497","provider":"danyel biotech","version":"1.0","type":"link"}