{"product_id":"masterpure-dna-purification-kit-for-blood-version-ii-mb711400","title":"MasterPure DNA Purification Kit for Blood Version II","description":"\u003cp\u003eThe MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimised protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes (Table 1). The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions (Figure. 1), for digestion with restriction enzymes (Figure. 2), for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and TNA purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.\u003c\/p\u003e\u003cp\u003eStarting Material\tYield\u003cbr\u003e600 µl Buffy Coat\t87-109 µg\u003cbr\u003e200 µl Whole Blood\t3-9 µg\u003cbr\u003e0.6 ml Whole Blood\t9-27 µg\u003cbr\u003e0.8 ml Whole Blood\t12-36 µg\u003cbr\u003e1 ml Whole Blood\t15-45 µg\u003cbr\u003e2 ml Whole Blood\t30-90 µg\u003cbr\u003e3 ml Whole Blood\t45-120 µg\u003cbr\u003e4 ml Whole Blood\t60-180 µg\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEasily purify high quality genomic DNA from whole blood or buffy coat.\u003c\/li\u003e\n\u003cli\u003eScalable purification of gDNA from blood\u003c\/li\u003e\n\u003cli\u003eConsistent yields of up to 10 µg of DNA from 325 µL of whole blood and approximately 100 µg DNA from 600 µL of buffy coat\u003c\/li\u003e\n\u003cli\u003eHigh purity gDNA with A260\/A280 ratios of 1.8-2.0 making the DNA suitable for most downstream molecular biology applications including PCR and sample archiving\u003c\/li\u003e\n\u003cli\u003eFast protocol completed in less than 30 minutes\u003c\/li\u003e\n\u003cli\u003eImproved yields by avoiding the use of columns\u003c\/li\u003e\n\u003cli\u003eBetter safety since no hazardous organic solvents are used\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, RNase A, TE Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_MB711400_MB711400-MasterPure-DNA-Purification-Kit-Blood-Version-II\"\u003eMB711400 MasterPure DNA Purification Kit Blood Version II\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MB711400_MasterPure-Blood\"\u003eMasterPure Blood\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MB711400_masterpure-dna-purification-kit-for-blood-version-ii\"\u003emasterpure dna purification kit for blood version ii\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986445393999,"sku":"MB711400","price":3946.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MasterPure-DNA-Purification-Kit-for-Blood-Version-II_26febac0-39dd-4a00-b00a-2a9a219b2c76.jpg?v=1785956302","url":"https:\/\/www.danyel.co.il\/products\/masterpure-dna-purification-kit-for-blood-version-ii-mb711400","provider":"danyel biotech","version":"1.0","type":"link"}