{"product_id":"masterpure-complete-dna-and-rna-purification-kit-naexpu-001","title":"MasterPure Complete DNA and RNA Purification Kit","description":"\u003cp\u003eThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.\u003c\/p\u003e\u003cp\u003eThe kit procedure uses a safe, gentle, and scalable salt-precipitation protocol (Figure. 1) that eliminates the need for hazardous chemicals and yield-reducing columns. Purification reactions can be scaled up or scaled down depending on the amount of nucleic acid needed and the size of the sample. MasterPure Kits for nucleic acid purification from Gram-positive bacteria, yeast (DNA or RNA), and blood samples are also available.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003ePurification of genomic DNA or total RNA for many applications, including:\u003c\/p\u003e\u003cp\u003eLibrary preparation for next generation sequencing (NGS) of genomic DNA and RNA\u003cbr\u003eDNA methylation studies using Illumina® Infinium® HumanMethylation BeadChips\u003cbr\u003eGenomic DNA and cDNA cloning\u003cbr\u003eqPCR and qRT-PCR\u003cbr\u003eMicrarray analyses (CGH, gene expression profiling, etc)\u003c\/p\u003e\u003cp\u003eFigure 1. Overview of the MasterPure Complete Kit protocol.\u003c\/p\u003e\u003cp\u003eFigure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 × 10 7 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.\u003c\/p\u003e\u003cp\u003eFigure 3. DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure Complete Kit. M = kb ladder.\u003c\/p\u003e\u003cp\u003eTargets\tSamples Extracted\u003c\/p\u003e\u003cp\u003eHuman \u0026amp; mammalian RNA \u0026amp; genomic DNA\u003cbr\u003eHIV\u003cbr\u003eHCV\u003cbr\u003eRSV\u003cbr\u003eHPV\u003cbr\u003eYeast\u003cbr\u003eEnterovirus\u003cbr\u003eB. pertussis\u003cbr\u003eE. coli\u003cbr\u003eM. tuberculosis\u003cbr\u003eStreptococcus mutans\u003cbr\u003eSoy (tofu)\u003cbr\u003eMaize\u003cbr\u003eInsect tissues\u003c\/p\u003e\u003cp\u003ePlasma\u003cbr\u003eWhole blood\u003cbr\u003eGuthrie cards\u003cbr\u003eBuccal cells\u003cbr\u003eLiver\u003cbr\u003eMouse tail\u003cbr\u003eKidney\u003cbr\u003eSaliva\u003cbr\u003eUrine\u003cbr\u003eSputum\u003cbr\u003eTissue-culture cell lines\u003cbr\u003eCervical cells\u003cbr\u003eParaffin-embedded tissues\u003c\/p\u003e\u003cp\u003eTable 1. Examples of targets and samples analysed using MasterPure DNA and RNA Purification Kits. Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.\u003c\/p\u003e\u003cp\u003eSample\tSample Size\tTNA µg\tDNA µg\tRNA µg\u003cbr\u003eHeLa\/HL60 cells\t1 × 10 6 cells\t10-30\t3-12\t7-15\u003cbr\u003eTissues\u003cbr\u003eLiver\t5 mg\t33-42\t5-10\t13-25\u003cbr\u003eBrain\t5 mg\t9-13\t6-9\t4-11\u003cbr\u003eHeart\t5 mg\t6-10\t4-7\t4-5\u003cbr\u003eKidney\t5 mg\t10-17\t3-8\t14-17\u003cbr\u003eThymus\t5 mg\t15-30\t6-12\t9-18\u003cbr\u003eBlood\t200 µl\t3-10\t3-9\t\u003cbr\u003eBuffy coat\t300 µl\t40-55\t40-55\t3-6\u003cbr\u003eMouse tail\t0.5 cm\t25-30\t9-11\t\u003cbr\u003eE. coli\t3.5 × 10 6 cells\t2.5-2.8\t1.3-1.6\t1.6-1.8\u003cbr\u003eS. mutans\t1.5 ml\t0.9\u003c\/p\u003e\u003cp\u003eYeast*\u003c\/p\u003e\u003cp\u003e( S. cerevisiae)\u003c\/p\u003e\u003cp\u003e2.2 × 10 6 cells\t\t\t11-18\u003cbr\u003e1.1 × 10 7 cells\t\t\t70-78\u003c\/p\u003e\u003cp\u003e*For extraction of yeast DNA, the MasterPure Yeast DNA Purification Kit is recommended.\u003c\/p\u003e\u003cp\u003eTable 2. Example of yields of nucleic acids obtained using MasterPure Complete DNA and RNA Purification Kits.\u003c\/p\u003e\u003cp\u003eFigure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure Complete DNA and RNA Purification Kit. Lane M, Kilobase ladder.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePurify Total Nucleic Acid (TNA), DNA or RNA in 30-60 minutes\u003c\/li\u003e\n\u003cli\u003eSafe: Does not use hazardous phenol, chloroform or guanidine\u003c\/li\u003e\n\u003cli\u003eHigh Purity: A260\/A280 ratios consistently between 1.8 and 2.0\u003c\/li\u003e\n\u003cli\u003eHigh Yields: Improves yields by avoiding the use of columns which often reduce nucleic acid yields\u003c\/li\u003e\n\u003cli\u003eVersatile: Purify TNA, genomic DNA, total RNA, FFPE RNA, or both genomic DNA and total RNA from a sample\u003c\/li\u003e\n\u003cli\u003eTotal RNA Recovery: Purify both large and small (e.g., miRNA) RNA for RNA-Seq or qRT-PCR\u003c\/li\u003e\n\u003cli\u003eProven: Hundreds of citations for purification of DNA and RNA from dozens of sample types for use in many applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_NAEXPU-001_MC85200-MC89010-MasterPure-Complete-DNA-RNA-Purification-Kit\"\u003eMC85200 MC89010 MasterPure Complete DNA RNA Purification Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NAEXPU-001_MasterPure-Complete\"\u003eMasterPure Complete\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NAEXPU-001_masterpure-complete-dna-and-rna-purification-kit\"\u003emasterpure complete dna and rna purification kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NAEXPU-001_masterpure-complete-dna-and-rna-purification-kit-bulk-reagents\"\u003emasterpure complete dna and rna purification kit bulk reagents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_NAEXPU-001_E101-US-MasterPure\"\u003eE101 US MasterPure\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"200 Purif","offer_id":45986445328463,"sku":"MC85200","price":3179.0,"currency_code":"ILS","in_stock":true},{"title":"10 Purif","offer_id":45986445361231,"sku":"MC89010","price":663.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MasterPure-Complete-DNA-and-RNA-Purification-Kit_4da54b28-b506-488e-95d0-299b2769e15f.jpg?v=1785956301","url":"https:\/\/www.danyel.co.il\/products\/masterpure-complete-dna-and-rna-purification-kit-naexpu-001","provider":"danyel biotech","version":"1.0","type":"link"}