{"product_id":"maize-gmo-mon863-qpcr-detection-tbs43005-100","title":"Maize GMO Mon863 qPCR Detection","description":"\u003cp\u003eMany countries in the world regulate the cultivation and trade with genetically modified organisms (GMO). The enforcement of such regulations depends on the ability to detect and quantify the presence of GMOs in food, feed, and seed products. The real-time qPCR method is sensitive and robust and is regarded as the gold standard for GMO analysis.\u003c\/p\u003e\n\u003cp\u003eThe Maize GMO MON863 qPCR Kit is designed for identifying GMO marker MON863 presence or absence in food, feed, and seed products using real-time quantitative polymerase chain reaction(qPCR), primers, and labeled probe. The Kit includes maize GMO MON863 Positive and Negative controls, and PCR internal controls, qPCR Super Mix, maize GMO MON863 Prime-Probe Mix, in which the probe is labeled with Fam, and Hex is labeled for PCR internal control. These aids in the straightforward interpretation of the results.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKEY FEATURES\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHighly sensitivity and specificity for identification of GMO MON863.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency: the optimal systemic conditions for PCR amplification.\u003c\/li\u003e\n\u003cli\u003eStreamlined protocol: Just add DNA Template and water.\u003c\/li\u003e\n\u003cli\u003eNo cross reactivity with others.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003ePCR PROTOCOL\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1. DNA extraction:\u003c\/strong\u003e The methods for DNA extraction can be used for any suitable preparation of DNA purification from food samples. We recommend that the Fast genomic DNA extraction method be used for this purpose (catalog: TBS6008).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Set up a PCR reaction for each sample in 20 µL\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 300px;height: 169px\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReaction Component\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eVolume (µL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eqPCR Super Mix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePrimer-probe Mix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eNuclease-free Water\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDNA sample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFinal Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eInternal control should be included as below: Positive Control or Negative control (5 µL \/reaction).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Suggested PCR conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 488px;height: 167px\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\"\u003e\u003cstrong\u003eStep\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAmplification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003e\u003cstrong\u003ePCR\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003e\u003cstrong\u003eHOLD\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eCYCLE (40 cycles)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDenature\u003c\/td\u003e\n\u003ctd\u003eAnneal\/ Extend\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTemperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e95 °C\u003c\/td\u003e\n\u003ctd\u003e95 °C\u003c\/td\u003e\n\u003ctd\u003e60 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTime\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 min\u003c\/td\u003e\n\u003ctd\u003e15 sec\u003c\/td\u003e\n\u003ctd\u003e60 sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRELATIVE PRODUCTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/nucleotide\/mouse-dna-extraction\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTBS6008: Fast Genomic DNA Extraction\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/nucleotide\/microbial-dna-magnetic-extraction-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTBS6025: Microbial DNA Magnetic Extraction\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/food-safety\/gmo-detections\/maize-gmo-98140-qpcr-detection\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTBS43001: Maize GMO 98140 qPCR Detection\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/food-safety\/gmo-detections\/maize-gmo-dbt418-qpcr-detection\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTBS43003: Maize GMO DBT418 qPCR Detection\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/food-safety\/gmo-detections\/maize-gmo-ly038-qpcr-detection\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTBS43004: Maize GMO LY038 qPCR Detection\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS43005-100--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/02\/TBS43005_Maize_GMO-MON863_qPCR-kit_V01_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/02\/TBS43005_Maize_GMO-MON863_qPCR-kit_MSDS-2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986986917967,"sku":"TBS43005-100","price":2090.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS43005-0.jpg?v=1782819252","url":"https:\/\/www.danyel.co.il\/products\/maize-gmo-mon863-qpcr-detection-tbs43005-100","provider":"danyel biotech","version":"1.0","type":"link"}