{"product_id":"mag-bind-ffpe-dna-rna-96-kit-m6955-00","title":"Mag-Bind® FFPE DNA\/RNA 96 Kit","description":"\u003cdiv class=\"product-summary\"\u003e\n\u003cem\u003e\u003cp\u003e\u003cstrong\u003eNew improved kit with a faster protocol!\u003c\/strong\u003e Sequential isolation of both DNA and RNA from the same FFPE sample using magnetic beads.\u003c\/p\u003e \u003c\/em\u003e \u003cul\u003e \u003cli\u003e \u003cdiv\u003e\n\u003cstrong\u003eDual Extraction from Same Sample\u003c\/strong\u003e – One protocol, two analytes—recover both DNA and RNA without sample splitting\u003c\/div\u003e \u003c\/li\u003e \u003cli\u003e \u003cdiv\u003e\n\u003cstrong\u003eSafe Workflow\u003c\/strong\u003e -Xylene-free deparaffinization\u003c\/div\u003e \u003c\/li\u003e \u003cli\u003e \u003cdiv\u003e\n\u003cstrong\u003eFaster processing\u003c\/strong\u003e – Reduced incubation times for faster turnaround\u003c\/div\u003e \u003c\/li\u003e \u003cli\u003e \u003cdiv\u003e\n\u003cstrong\u003eAutomation-Ready for High-Throughput\u003c\/strong\u003e – Compatible with leading open ended automation platforms (e.g., KingFisher™, Hamilton®)\u003c\/div\u003e \u003c\/li\u003e \u003cli\u003e \u003cdiv\u003e\n\u003cstrong\u003eConsistent and Reliable Performance\u003c\/strong\u003e – Supports NGS, qPCR, and other downstream workflows\u003c\/div\u003e \u003c\/li\u003e \u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eMag-Bind® FFPE DNA\/RNA 96 Kit is designed for the sequential isolation of DNA and RNA in separate eluates from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The purification of DNA and RNA from the same sample enables a more comprehensive analysis from a precious sample source. The protocol utilizes a specially formulated buffer system that not only partially reverses the formaldehyde-induced crosslinking but also ensures DNA and RNA are differentially purified with no cross-contamination. The kit can also be used for purification of only DNA or only RNA from FFPE samples if sequential\u003cbr\u003eisolation is not desired. Magnetic bead-based extraction makes it suitable for both manual as well as automated processing. Purified DNA and RNA are suitable for a variety of downstream applications including SNP analysis, next generation sequencing, and genotyping.\u003c\/p\u003e\n\u003cp\u003eThe Mag-Bind® FFPE DNA\/RNA 96 Kit integrates a unique buffer system with the highly efficient binding properties of Mag-Bind® technology to isolate total DNA and RNA in separate eluates from the same FFPE sample. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample eliminating the use of hazardous xylene.\u003c\/p\u003e\n\u003cp\u003eSamples are first lysed in FDR Buffer aided by the presence of Proteinase K enzyme. The lysate is then heated to denature the proteinase and reverse the chemical crosslinking of the nucleic acids. Post-heating, the lysate is mixed with MB4 Buffer and Mag-Bind® Particles CH to bind DNA to the particles. The RNA-containing supernatant is saved and a second binding step is completed with addition of isopropanol to bind RNA to the Mag-Bind® Particles CH. This results in separation of DNA and RNA into two fractions. Mag-Bind® Particles bound to DNA and RNA are individually washed and nucleic acids are eluted in two different tubes for further analyses. Only DNA or RNA can be isolated following the appropriate protocols outlined in the manual.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. If automating this procedure on a liquid handler or a magnetic processor, please contact your Omega Bio-tek representative for instrument-specific instructions.\u003cbr\u003e2. This kit includes enough reagents for the specified number of preparations plus at least an additional 10% overage to ensure there is sufficient volume. Please be aware that the actual number of preparations may be lower due to pre-aliquoting\u003cbr\u003eof reagents, processing partial plates, and automation platform used etc. Additional reagents are available for purchase separately. \u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Applications\u003c\/th\u003e\n\u003ctd\u003eNGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e50-200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eFFPE tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 3 FFPE sections of 10 μm thickness each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003eUp to 96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Mode\u003c\/th\u003e\n\u003ctd\u003eAutomated, Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTechnology\u003c\/th\u003e\n\u003ctd\u003eMagnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.4203.S2R4E1A3.20190624.67.4684394\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.11076.S2R4E1A3.20230907.294.5158472\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6783.S2R4E1A3.20210528.8.4465142\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"1 X 96 Preps","offer_id":45981763305551,"sku":"M6955-00","price":3504.0,"currency_code":"ILS","in_stock":true},{"title":"4 X 96 Preps","offer_id":45981763338319,"sku":"M6955-01","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MagneticBeads-M6955-M2511-M6958-copy.webp?v=1782734853","url":"https:\/\/www.danyel.co.il\/products\/mag-bind-ffpe-dna-rna-96-kit-m6955-00","provider":"danyel biotech","version":"1.0","type":"link"}