{"product_id":"mag-bind-bacterial-dna-96-kit-m2350-00","title":"Mag-Bind® Bacterial DNA 96 Kit","description":"\u003cdiv class=\"product-summary\"\u003e\n\u003cp\u003eIsolate DNA from up to gram positive and negative bacteria\u003c\/p\u003e \u003cul\u003e \u003cli\u003eReliable – Reproducible DNA purification from a variety of sample sources\u003c\/li\u003e \u003cli\u003eHigh yield – Enzymatic digestion for cell lysis\u003c\/li\u003e \u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe Mag-BIND® Bacterial DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0.5 mL gram positive or gram negative bacterial culture can be processed each time. The key to the system is Omega Bio-tek’s proprietary Mag-BIND® particles that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. After bacterial cells are collected from culture or picked from an agar plate, the bacterial cell wall is removed by lysozyme digestion, followed by Proteinase K digestion. Following lysis, binding conditions are adjusted and the sample is mixed with Mag-BIND particles to bind DNA. Three rapid wash steps remove trace salts and protein contaminants, and finally, DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eWide variety of bacterial species\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTechnology\u003c\/th\u003e\n\u003ctd\u003eMagnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Mode\u003c\/th\u003e\n\u003ctd\u003eAutomated, Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNote\u003c\/th\u003e\n\u003ctd\u003eCH beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2401.S2R4E1A3.20190214.67.4662130\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6785.S2R4E1A3.20210528.8.4468772\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"1 X 96 Preps","offer_id":45981761241167,"sku":"M2350-00","price":2677.0,"currency_code":"ILS","in_stock":true},{"title":"4 X 96 Preps","offer_id":45981761273935,"sku":"M2350-01","price":6104.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MagneticBeads-M2350-copy-1.webp?v=1782734849","url":"https:\/\/www.danyel.co.il\/products\/mag-bind-bacterial-dna-96-kit-m2350-00","provider":"danyel biotech","version":"1.0","type":"link"}