{"product_id":"lipoxidase-enzyme-activity-tbp0079","title":"Lipoxidase, Enzyme Activity","description":"\u003cp\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Readily soluble in distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e -20° C; -4° F\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 10 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 60%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOne unit will cause an increase in A232.5 of 0.001 per minute at pH 9.0 at 25°C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A232.5 unit is equivelent to the oxidation of 0.12 µmol of linoleic acid.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLipoxidase (EC 1.13.11.12) catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides. The degradation of the hydroperoxide by the lipoxidase seems to prevent the breakdown of amino acids and proteins which are associated with an odorous carbyl compound which produces the beany flavor found in many legumes.\u003c\/p\u003e\n\u003cp\u003eLipoxidase is widely distributed, especially concentrated in the legumes. Soybeans have been found to have the highest concentrations while isoenzymes have been reported in cowpeas.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M ammonium hydroxide ammonium chloride buffer, pH 9.0\u003c\/li\u003e\n\u003cli\u003e1 mM Linoleic acid\u003c\/li\u003e\n\u003cli\u003e20 µl Lipoxydase diluted to 0.6 U\/ml\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with a strip chart recorder and temperature control) at 232.5 nm and 25°C\u003c\/li\u003e\n\u003cli\u003eInto the quartz cuvettes pipette 2.9 ml ammonium hydroxide ammonium chloride buffer\u003c\/li\u003e\n\u003cli\u003eAdd 0.1ml of the enzyme solution to the test cuvette, mix, and record the rate of absorbance at 232.5 nm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate the ÆE232.5nm\/min from the initial linear portion of the curve.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0079-1000KU TBP0079-5000KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0079-Lipoxidase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0079_Lipoxidase-Dehydrogenase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1000 KU","offer_id":45986499985487,"sku":"TBP0079-1000KU","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5000 KU","offer_id":45986500018255,"sku":"TBP0079-5000KU","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0079_3bc22dd0-97a2-4f4c-9684-768aa5eb7ed4.png?v=1782812664","url":"https:\/\/www.danyel.co.il\/products\/lipoxidase-enzyme-activity-tbp0079","provider":"danyel biotech","version":"1.0","type":"link"}