{"product_id":"leuko-spin-medium-density-gradient-medium-pluriselect","title":"Leuko Spin Medium, Density Gradient Medium","description":"\u003cp\u003eLeuko Spin density gradient medium is a sterile, ready-to-use solution for the isolation of human leukocytes from fresh sample material (whole blood or buffy coat) with high yield. Mononuclear and polymorphonuclear (polymorphonuclear) cells can be enriched with Leuko Spin Medium using a simple density gradient centrifugation. In combination with PBMC Spin Density Medium, themononuclear and polymorphonuclear cell fractions can be enriched simultaneously from one sample.\u003c\/p\u003e\u003ch2\u003eFeatures\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady to use\u003c\/strong\u003e – The pluriSpin separation media are ready to use and can be used directly for cell separation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReplace\u003c\/strong\u003e – Alternative products, such as GranuloSep, Pancoll or Histopaque - 1119, for the enrichment of leukocytes can be substituted with Leuko Spin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible\u003c\/strong\u003e – The enriched cells can be used for downstream applications such as MACS, SepMate, RosetteSep, MojoSort.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCombinable\u003c\/strong\u003e – The pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSeparation Scheme\u003c\/h2\u003e\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-diagramm-v3.webp\" alt=\"Leuko Spin Medium, Density Gradient Medium separation scheme\" style=\"max-width:100%\"\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDistribution of blood cell populations as a function of density\u003c\/strong\u003e\u003cbr\u003eBlood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.\u003c\/p\u003e\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-diagramm-leuko-spin-v2.webp\" alt=\"Leuko Spin Medium, Density Gradient Medium separation scheme\" style=\"max-width:100%\"\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget cell population with Leuko Spin density centrifugation\u003c\/strong\u003e\u003cbr\u003eThe density of the separation media defines the cut-off for the cells. Leuko Spin Medium has a cut-off of 1.090 g\/ml. All cells with a lower density, such as thrombocytes (1), monocytes (2), lymphocytes (3), basophil granulocytes (4), neutrophil granulocytes (5) are enriched with Leuko Spin Medium. All cells with a higher density, such as eosinophil granulocytes (6) and erythrocytes (7), pass through the Leuko Spin medium and are depleted. First, the sample material is carefully layered onto the Leuko Spin density medium. Mixing of the two phases (sample material and density medium) must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The two layers of leukocytes are then transferred to a new tube and the cells are washed.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBEFORE\u003c\/strong\u003e\u003cbr\u003ePreparation of leukocytes with Leuko Spin Medium. The sample material (1) is carefully placed on the Leuko Spin Medium (2).\u003c\/p\u003e\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-centrifugation-leuko-spin-2-v1.webp\" alt=\"Leuko Spin Medium, Density Gradient Medium separation scheme\" style=\"max-width:100%\"\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAFTER\u003c\/strong\u003e\u003cbr\u003eLayers after centrifugation. After centrifugation, the leukocytes form two white layers on the Leuko Spin Medium (4). Layer (2) consists of mononuclear leukocytes and layer (3) of polymorphonuclear leukocytes (granulocytes). Cells with a higher density, such as erythrocytes, and dead cells pass through the medium and are located at the bottom of the tube (4).\u003c\/p\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSterility\u003c\/th\u003e\n\u003ctd\u003esterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell population\u003c\/th\u003e\n\u003ctd\u003eLeucocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation platform\u003c\/th\u003e\n\u003ctd\u003enon-magnetic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation method\u003c\/th\u003e\n\u003ctd\u003enegative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct type\u003c\/th\u003e\n\u003ctd\u003eCell separation reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReagent type\u003c\/th\u003e\n\u003ctd\u003eDensity gradient medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eDensity gradient centrifugation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTerms of delivery\u003c\/th\u003e\n\u003ctd\u003eambient temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage condition\u003c\/th\u003e\n\u003ctd\u003eroom temperature, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRegulatory statement\u003c\/th\u003e\n\u003ctd\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget cells\u003c\/th\u003e\n\u003ctd\u003eall leukocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSeparation density cut-off\u003c\/th\u003e\n\u003ctd\u003e1,090 g\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample material\u003c\/th\u003e\n\u003ctd\u003ehuman whole blood, Buffy Coat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatibility with\u003c\/th\u003e\n\u003ctd\u003epluriMate, SepMate, LeucoSep\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCan be used with\u003c\/th\u003e\n\u003ctd\u003epluriSpin, MACS, EasySep, RosetteSep, MojoSort\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003cth\u003ePacking\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00091-10\u003c\/td\u003e\n\u003ctd\u003e100 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00091-11\u003c\/td\u003e\n\u003ctd\u003e250 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e60-00091-12\u003c\/td\u003e\n\u003ctd\u003e500 ml\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"pluriSelect","offers":[{"title":"100 ml","offer_id":68092638658639,"sku":"60-00091-10","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"250 ml","offer_id":68092638691407,"sku":"60-00091-11","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"500 ml","offer_id":68092638724175,"sku":"60-00091-12","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-product-285.jpg?v=1794313282","url":"https:\/\/www.danyel.co.il\/products\/leuko-spin-medium-density-gradient-medium-pluriselect","provider":"danyel biotech","version":"1.0","type":"link"}