{"product_id":"lenti-x-packaging-single-shots-ecotropic-631278","title":"Lenti-X™ Packaging Single Shots (Ecotropic)","description":"\u003cp\u003eLenti-X Packaging Single Shots (Ecotropic) provide a one-step production method to generate high-titer lentivirus. Each tube of Lenti-X Packaging Single Shots (Ecotropic) contains premeasured, lyophilized Xfect Transfection Reagent and an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer ecotropic lentivirus is produced by adding the lentiviral vector of choice in sterile water to the tube, reconstituting, and then using the mixture to transfect 293T cells (e.g., Lenti-X 293T Cell Line, Cat. # 632180) in a 10-cm dish.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLenti-X Packaging Single Shots (VSV-G) and Lenti-X Packaging Single Shots (96-well, VSV-G) provide the highest titers (107–108 IFU per ml), give the broadest tropism, and are provided in single-tube and 96-well formats, respectively\u003c\/li\u003e\n\u003cli\u003eLenti-X Packaging Single Shots (Ecotropic) restrict tropism to cells of mouse and rat origin\u003c\/li\u003e\n\u003cli\u003eLenti-X Packaging Single Shots (Integrase Deficient) produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate into the host cell genome\u003c\/li\u003e\n\u003cli\u003eLenti-X Packaging Single Shots (Envelope-Free) enable the flexiblity to produce lentivirus pseudotyped with an envelope protein of choice\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGene delivery to hard-to-transfect cell types\u003cbr\u003eCreation of stable cell lines\u003cbr\u003eStreamlined creation of VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus\u003cbr\u003eCreation of lentivirus pseudotyped with an envelope protein of choice for use in serological assays\u003cbr\u003eHigh-throughput production of lentiv\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/631278\/631278.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/631278\/631278-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Lenti\/Lenti-X%20Packaging%20Single%20Shots%20Protocol-At-A-Glance_070618.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"16 Rxns","offer_id":45986024816719,"sku":"631278","price":6253.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/631278-Lenti-X-Packaging-Single-Shots-Ecotropic-product-600.jpg?v=1785957344","url":"https:\/\/www.danyel.co.il\/products\/lenti-x-packaging-single-shots-ecotropic-631278","provider":"danyel biotech","version":"1.0","type":"link"}