{"product_id":"ldh-lactate-dehydrogenase-colorimetric-assay-tbs2012","title":"LDH Lactate Dehydrogenase Colorimetric Assay","description":"\u003cp\u003eLactate dehydrogenase (LDH) is a cytosolic enzyme present in nearly all living cells. It is an oxidoreductase catalyzing the interconversion of lactate and pyruvate. LDH is released upon damage of the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate tissue or cell damage and cell cytotoxicity.\u003c\/p\u003e\n\u003cp\u003eThe Tribo™ LDH Colorimetric Assay Kit uses WST-8, which is oxidized to generate formazan to measure the activity of LDH released from damaged cells. The generated signal at OD 460 nm is proportional to the LDH released from the lysed cells. The kit provides the easiest and most accurate approach to measure LDH activity.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 1: LDH Assay Principle\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-15277 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2012-2.png\" alt=\"\" width=\"464\" height=\"155\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eQuantify lactate dehydrogenase in a variety of samples such as serum, plasma tissues, cells, and cell culture medium.\u003c\/li\u003e\n\u003cli\u003eThis kit is designed in a format for a variety of sample types. To measure LDH released into a cell culture medium in a cytotoxicity experiment, we recommend TBS2002, LDH Cytotoxicity Colorimetric Assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the Reagent at –20°C protected from light. Shelf life: 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 2: LDH Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-15278 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2012-3.png\" alt=\"\" width=\"360\" height=\"304\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Products\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/resazurin-cell-viability-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eResazurin Cell Viability Kit (TBS2001)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/wst-8-or-cell-count-kit-8cck-8\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eCCK-8 Cell Viability Assay (TBS2022)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/atp-colorimetric-fluorometric-assay-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eATP Colorimetric\/Fluorometric Assay Kit (TBS2010)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/adp-atp-ratio-assay-kitbioluminescent\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eADP\/ATP Ratio Assay Kit (Bioluminescent (TBS2015)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/adp-colorimetric-fluorometric-assay-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eADP Colorimetric\/Fluorometric Assay Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2012-LDH-Activity-Colorimetric-Assay-kit-7-2023.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2012-LDH-Activity-Colorimetric-Assay-kit_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986420097103,"sku":"TBS2012","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2012.jpg?v=1782810741","url":"https:\/\/www.danyel.co.il\/products\/ldh-lactate-dehydrogenase-colorimetric-assay-tbs2012","provider":"danyel biotech","version":"1.0","type":"link"}