{"product_id":"in-fusion-snap-assembly-ecodry-master-mix-with-competent-cells-638953","title":"In-Fusion® Snap Assembly EcoDry™ Master Mix with Competent Cells","description":"\u003cp\u003eThe In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEase of use and consistency—Lyophilized, pre-aliquoted cloning mix; dried-down format eliminates handling errors and contamination, facilitating high-throughput workflows\u003c\/li\u003e\n\u003cli\u003eHighly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb\u003c\/li\u003e\n\u003cli\u003eSub-cloning is unnecessary—Clone any insert, into any locus, in any vector, with just one reaction\u003c\/li\u003e\n\u003cli\u003eSeamless construction—Final constructs have no superfluous base pairs (as is often the case with restriction digest or TA cloning)\u003c\/li\u003e\n\u003cli\u003eVersatility and flexibility for your applications—Clone single or multiple DNA fragments simultaneously, or perform site-directed mutagenesis in a single reaction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/638953\/638953.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST2319\/ST2319-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/In\/In-Fusion%20Snap%20Assembly%20EcoDry%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"96 Rxns","offer_id":45986090156111,"sku":"638953","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/638953-In-Fusion-Snap-Assembly-EcoDry-Master-Mix-with-Competent-Cells-product-600.jpg?v=1782803775","url":"https:\/\/www.danyel.co.il\/products\/in-fusion-snap-assembly-ecodry-master-mix-with-competent-cells-638953","provider":"danyel biotech","version":"1.0","type":"link"}