{"product_id":"hydragel-csf-isofocusing-oligoclonal-igg-bands-kit-cns-local-igg-synthesis-4353","title":"HYDRAGEL CSF ISOFOCUSING Oligoclonal IgG Bands Kit (CNS Local IgG Synthesis)","description":"\u003cp\u003e\u003cstrong\u003eThe gold standard assay to objectively detect CNS local IgG synthesis in multiple sclerosis.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003ePresence of intrathecal IgG suggests inflammatory disease of the Central Nervous System, such as caused by multiple sclerosis (MS).\u003c\/p\u003e \u003cp\u003eThe HYDRAGEL CSF ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection and immunological characterization of IgG oligoclonal banding in human CSF (Cerebro Spinal Fluid) and serum. The semi-automated HYDRASYS instrument enables migration, immunofixation and direct detection on agarose gels, in order to obtain IgG profiles for qualitative analysis.\u003c\/p\u003e \u003cp\u003eThe main recommendations of European Consensus Report for Multiple Sclerosis diagnosis are:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eIsoelectrofocusing (IEF) on Agarose gel for immunoglobulins separation\u003c\/li\u003e \u003cli\u003eUse of non-concentrated CSF material\u003c\/li\u003e \u003cli\u003eSimultaneous analysis of the patient’s serum and CSF\u003c\/li\u003e \u003cli\u003eIdentical IgG concentration in paired CSF-serum samples\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThe presence of CSF oligoclonal bands only, characterizing local IgG synthesis, tightly correlates with the diagnosis of Multiple Sclerosis. Their presence provides useful information when in agreement with the other clinical investigations.\u003c\/p\u003e\u003ch2\u003eKey Benefits\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccurate Diagnosis of inflammatory disease of the CNS:\u003c\/strong\u003e Sensitivity increased about a 100-fold compared to standard immunofixation thanks to the detection with enzyme labelled antibodies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEfficient Method:\u003c\/strong\u003e Clear oligoclonal band visualization for a reliable interpretation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFaster result:\u003c\/strong\u003e Simple step-by-step procedure without membrane transfer for 3 hours of turnaround time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompliance with international consensus:\u003c\/strong\u003e HYDRAGEL CSF ISOFOCUSING meets all requirements needed to objectively detect CNS local IgG synthesis in multiple sclerosis.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eMain Characteristics\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThe HYDRAGEL CSF ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation. The patient’s serum and CSF samples are analysed in parallel to compare the IgG distribution.\u003c\/p\u003e \u003cul\u003e \u003cli\u003ePrior to performing the test, it is mandatory to quantify the IgG’s in both liquids, in order to adjust the IgG concentration at the same level in CSF and serum.\u003c\/li\u003e \u003cli\u003eAfter the protein separation according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-IgG antiserum, with no need for a membrane blotting step.\u003c\/li\u003e \u003cli\u003eThen, the gel is processed to remove unbound proteins before the enzymatic reaction.\u003c\/li\u003e \u003cli\u003eFinally, the gel is visually evaluated.\u003c\/li\u003e \u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSample Type\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eSerum and CSF samples are performed in parallel for each patient to compare IgG distribution as recommended by the European consensus.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePerformances\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e \u003cli\u003e2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result\u003c\/li\u003e \u003cli\u003eEnhanced software with high voltage monitoring\u003c\/li\u003e \u003cli\u003eFull traceability and positive ID obtained by connecting the HYDRASYS instrument to the ASSIST pipetting station\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eTests per gel\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e4353\u003c\/td\u003e\n\u003ctd\u003eHYDRAGEL 3 CSF ISOFOCUSING\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4355\u003c\/td\u003e\n\u003ctd\u003eHYDRAGEL 9 CSF ISOFOCUSING\u003c\/td\u003e\n\u003ctd\u003e9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Sebia","offers":[{"title":"3","offer_id":67629801734223,"sku":"4353","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"9","offer_id":67629801766991,"sku":"4355","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/zoom_gel_web_473x473_4a77dc63-6224-4249-b43f-a14bca1d52cc.png?v=1791291281","url":"https:\/\/www.danyel.co.il\/products\/hydragel-csf-isofocusing-oligoclonal-igg-bands-kit-cns-local-igg-synthesis-4353","provider":"danyel biotech","version":"1.0","type":"link"}