{"product_id":"hyclone-cell-boost-5-supplement-sh31117-02","title":"HyClone™ Cell Boost™ 5 Supplement","description":"\u003cp\u003eSupplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned to support modern manufacturing processes, Cell Boost™ 5 is chemically defined (CD), animal-derived component-free (ADCF), protein-free, and hydrolysate-free.\u003c\/li\u003e\n\u003cli\u003eFor convenience, this cell culture supplement is available as a ready-to-use liquid feed solution in addition to a dry powder format.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eImprove productivity and cell yield in fed-batch cultures by supplementing the cell culture medium to replace nutrients depleted during cell culture. HyClone™ Cell Boost™ 5 Supplement provides lipids, amino acids, vitamins, and growth factors to support fed-batch cultures in research and bioprocessing applications. It is available as a convenient ready-to-use liquid feed solution or as a dry powder feed to be hydrated before use.\u003c\/p\u003e\n\n\u003cp\u003eFed-batch applications\u003c\/p\u003e\n\n\u003cp\u003eThis HyClone™ cell culture supplement has been developed for recombinant protein production with various cell lines including Chinese hamster ovary (CHO) cell line, human embryonic kidney (HEK) 293, NS0, and hybridoma cells.\u003c\/p\u003e\n\n\u003cp\u003eGeneral culture recommendations\u003c\/p\u003e\n\n\u003cp\u003eRecommended supplementation range is between 20 to 200 mL\/L during mid to late growth phase. It is anticipated that this will be day 3 to 4 of the culture. It is suggested that L-glutamine is monitored separately in those cultures where it is present in the culture medium and supplemented as needed.\u003c\/p\u003e\n\n\u003cp\u003eDry powdered medium milling equivalency study\u003c\/p\u003e\n\n\u003cp\u003eThis study \u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/media-powder-milling-study\"\u003ecompares pin milling of powdered HyClone™ cell culture media and supplements\u003c\/a\u003e at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17565-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29728534.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"100 g","offer_id":45982134435919,"sku":"SH30865.01","price":1073.0,"currency_code":"ILS","in_stock":true},{"title":"1000 mL","offer_id":45982134468687,"sku":"SH31117.02","price":902.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-50057-original-png_540f86ac-e467-4971-8fcb-9f6d78302685.png?v=1785958667","url":"https:\/\/www.danyel.co.il\/products\/hyclone-cell-boost-5-supplement-sh31117-02","provider":"danyel biotech","version":"1.0","type":"link"}