{"product_id":"hyclone-cell-boost-3-supplement-sh31115-02","title":"HyClone™ Cell Boost™ 3 Supplement","description":"\u003cp\u003eSupplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned to support modern manufacturing processes, Cell Boost™ 3 is chemically defined (CD), animal-derived component-free (ADCF), protein-free, and hydrolysate-free.\u003c\/li\u003e\n\u003cli\u003eFor convenience, this cell culture supplement is available as a ready-to-use liquid solution in addition to a dry powder format.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eImprove productivity and cell yield in fed-batch cultures by supplementing the cell culture medium to replace nutrients depleted during cell culture. HyClone™ Cell Boost™ 3 Supplement provides amino acids, vitamins, glucose, and trace elements to support fed-batch cultures in research and bioprocessing applications. It is available as a convenient ready-to-use liquid feed solution or as a dry powder feed to be hydrated before use.\u003c\/p\u003e\n\n\u003cp\u003eFed-batch applications\u003c\/p\u003e\n\n\u003cp\u003eThis HyClone™ cell culture supplement has been developed for recombinant protein production with various cell lines including hybridoma and myeloma cell lines.\u003c\/p\u003e\n\n\u003cp\u003eGeneral culture recommendations\u003c\/p\u003e\n\n\u003cp\u003eSupplement 20 to 200 mL\/L one or more times, starting during mid to late growth phase. This is expected to be day 3 to 4. Start the culture at a reduced volume according to anticipated feed volumes. Continue supplementation daily until the final volume is reached. It is recommended to monitor L- glutamine separately and supplement as needed.\u003c\/p\u003e\n\n\u003cp\u003eDry powdered medium milling equivalency study\u003c\/p\u003e\n\n\u003cp\u003eThis study \u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/media-powder-milling-study\"\u003ecompares pin milling of powdered HyClone™ cell culture media and supplements\u003c\/a\u003e at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17568-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29728532.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"100 g","offer_id":45982134337615,"sku":"SH30825.01","price":755.0,"currency_code":"ILS","in_stock":true},{"title":"1000 mL","offer_id":45982134370383,"sku":"SH31115.02","price":655.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-50055-original-png_6f648e0c-6af2-4c09-b902-5dfc2424b2b4.png?v=1785958664","url":"https:\/\/www.danyel.co.il\/products\/hyclone-cell-boost-3-supplement-sh31115-02","provider":"danyel biotech","version":"1.0","type":"link"}