{"product_id":"hybridase-thermostable-rnase-h-pcramp-022","title":"Hybridase Thermostable RNase H","description":"\u003cp\u003eHybridase Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA. In contrast to E. coli RNase H, which is rapidly inactivated at 55°C, Hybridase RNase H is active at high temperatures. It has optimal activity above 65°C and can be used at temperatures up to 95°C. The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridisation stringency for specific DNA:RNA heteroduplexes, maximizing sensitivity and selectivity while minimizing background due to nonspecific hybridization.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eHigh-stringency hybrid selection.\u003cbr\u003eDiagnostic assays of specific target DNA sequences by isothermal probe amplification.\u003cbr\u003eTranscription-based amplification methods (e.g.., NASBA® method).\u003cbr\u003eHigh-stringency mapping of mRNA structure.\u003cbr\u003eApplications that require specific hydrolysis of the RNA in a DNA:RNA hybrid.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSpecifically degrade the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridised RNA, at higher reaction temperatures\u003c\/li\u003e\n\u003cli\u003eOptimal activity above 65°C and maintains activity as high as 95°C\u003c\/li\u003e\n\u003cli\u003eHighly specific for RNA in a RNA:DNA hybrid and will not digest free RNA or DNA\u003c\/li\u003e\n\u003cli\u003eMaximises digestion sensitivity and selectivity while minimising background due to nonspecific hybridisation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Hybridase RNase H results in the acid-solubilization of 1 nmol of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eNote: The unit assay is performed at 45°C because this is optimal for the T m of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eQuality Control: Hybridase Thermostable RNase H is tested for RNA degradation in a RNA:DNA hybrid and for the absence of detectable exo- or endodeoxyribonuclease, and non-RNase H RNase activities.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_PCRAMP-022_Hybridase-Thermostable-RNase-H\"\u003eHybridase Thermostable RNase H\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-022_Hybridase-Thermostable-RNaseH\"\u003eHybridase Thermostable RNaseH\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"5 U\/µL, 500 U","offer_id":45986441822287,"sku":"H39500","price":2616.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 5000 U","offer_id":45986441855055,"sku":"H39500-1ML","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Hybridase-Thermostable-RNase-H_89bee59d-9688-4454-960e-eba8002ac980.jpg?v=1785956281","url":"https:\/\/www.danyel.co.il\/products\/hybridase-thermostable-rnase-h-pcramp-022","provider":"danyel biotech","version":"1.0","type":"link"}