{"product_id":"human-tnf-alpha-elisa-kit-tbs3235","title":"Human TNF-Alpha ELISA Kit","description":"\u003cp\u003eThe Tribo\u003csup\u003eTM\u003c\/sup\u003e Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours instead of 4-5 hours (Fig. 1). \u003cstrong\u003eThe detection range is from 8 to 2000 pg\/mL\u003c\/strong\u003e. The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNovel Proprietary ELISA Approach\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e Only 1 hour,  not 4-5 hrs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e Sample and Detection combined into one step.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEasy: \u003c\/strong\u003eAll procedures are performed at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad Detection Range:\u003c\/strong\u003e The detection range is from 15 to 1000 pg\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human TNF-α is typically 10 pg\/ml. The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human TNF-α.\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity: human TNF-β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF-α; Mouse TNF RI, Mouse TNF RII.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-9536 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-0.png\" alt=\"\" width=\"668\" height=\"136\" data-lazy-sizes=\"auto, (max-width: 668px) 100vw, 668px\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-9537 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-1.png\" alt=\"\" width=\"685\" height=\"366\" data-lazy-sizes=\"auto, (max-width: 685px) 100vw, 685px\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-9538 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-2.png\" alt=\"\" width=\"411\" height=\"330\" data-lazy-sizes=\"auto, (max-width: 411px) 100vw, 411px\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 381px\" border=\"1px solid black\" width=\"1261\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TNF-α Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS3235A\u003c\/td\u003e\n\u003ctd\u003e96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human TNF-α.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TNF-α Standard\u003c\/td\u003e\n\u003ctd\u003eTBS3235B\u003c\/td\u003e\n\u003ctd\u003e30 µl of Recombinant human TNF-α protein (100 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3235C\u003c\/td\u003e\n\u003ctd\u003e2.1 ml of Biotin-Human TNF-α antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3235D\u003c\/td\u003e\n\u003ctd\u003e12 ml of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3235E\u003c\/td\u003e\n\u003ctd\u003e12 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3235--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-human-TNFa-V04_03_2022-data-sheet.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235_human_TNF-Alpha_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003eAo, Z., Wu, Z., Cai, H., Hu, L., Li, X., Kaurich, C., Chang, J., Gu, M., Cheng, L., Lu, X., \u0026amp; Guo, F. (2022). Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters. \u003cem\u003eAdvanced science (Weinheim, Baden-Wurttemberg, Germany)\u003c\/em\u003e, \u003cem\u003e9\u003c\/em\u003e(22), e2201478. \u003ca href=\"https:\/\/doi.org\/10.1002\/advs.202201478\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1002\/advs.202201478\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986380316751,"sku":"TBS3235","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3235_TNFa.jpg?v=1782809954","url":"https:\/\/www.danyel.co.il\/products\/human-tnf-alpha-elisa-kit-tbs3235","provider":"danyel biotech","version":"1.0","type":"link"}