{"product_id":"human-nicotinamide-n-methyltransferase-nnmt-recombinant-protein-bioactive-tbp0002-20ug","title":"Human Nicotinamide N-Methyltransferase \/ NNMT Recombinant Protein, Bioactive","description":"\u003cp\u003eNNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta, and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for the biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli, and purified by using chromatography. This enzyme is validated for NNMT activity application.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpression System:\u003c\/strong\u003e E. coli.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDomain:\u003c\/strong\u003e 1-264aa.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUniProt No:\u003c\/strong\u003e P40261.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Nicotinamide N-methyltransferase\/ NNMT.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eProduct Specification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 37.7 kDa (284aa) confirmed by MALDI-TOF\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1mg\/ml (determined by Bradford assay).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation:\u003c\/strong\u003e Liquid in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, No DTT.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt; 95% by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTag:\u003c\/strong\u003e His-Tag.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiological Activity:\u003c\/strong\u003e Specific activity is \u0026gt; 100nmol\/min\/mg, and is defined as the amount of enzyme that transfers 1.0nmole of methyl group per minute at 37℃.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Condition:\u003c\/strong\u003e Store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme Activity, and protein assay by SDS-PAGE\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c!--TBP0002-20UG TBP0002-100UG TBP0002-500UG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/01\/TBP0002-NNMT-Recombinant-Protein-data-sheet_V01_2023.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/01\/TBP0002_Human-Nicotinamide-N-Methyltransferase-NNMT-Recombinant-Protein-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"20ug","offer_id":45986492088399,"sku":"TBP0002-20UG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"100ug","offer_id":45986492121167,"sku":"TBP0002-100UG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"500 ug","offer_id":45986492153935,"sku":"TBP0002-500UG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0002.png?v=1782812509","url":"https:\/\/www.danyel.co.il\/products\/human-nicotinamide-n-methyltransferase-nnmt-recombinant-protein-bioactive-tbp0002-20ug","provider":"danyel biotech","version":"1.0","type":"link"}