{"product_id":"human-insulin-elisa-1-step-and-1hr-tbs3236","title":"Human Insulin ELISA; 1 step and 1hr","description":"\u003cp\u003eTribioscience’s Fast Human Insulin ELISA is designed to quantitatively detect human insulin levels in different tissues including skin, muscle, neural, serum, and other biological samples. \u003cspan style=\"color: #3366ff\"\u003eThe main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 4-5 hours (Fig. 1). The detection range is from 51.2 to 12500 pg\/mL.\u003c\/span\u003e The levels of human insulin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human insulin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human is typically 20pg\/ml. The Intra-assay CV is 4.79% the Inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human insulin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11200 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3236-1.png\" alt=\"\" width=\"616\" height=\"202\" data-lazy-sizes=\"auto, (max-width: 616px) 100vw, 616px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11201 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3236-2.jpg\" alt=\"\" width=\"733\" height=\"397\" data-lazy-sizes=\"auto, (max-width: 733px) 100vw, 733px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11202 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3236-3.png\" alt=\"\" width=\"461\" height=\"321\" data-lazy-sizes=\"auto, (max-width: 461px) 100vw, 461px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 85%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"61\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"276\"\u003e\n\u003cstrong\u003eSTORAGE OF \u003c\/strong\u003e\u003cstrong\u003eOPENED\/ \u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman Insulin Microplate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3236A\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human insulin.\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman Insulin Standard\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3236B\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e100 µl of Recombinant human insulin (100ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3236C\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e2.2 ml of Biotin- human insulin antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"276\"\u003e \n\u003cp\u003eMay be stored for up to\u003c\/p\u003e\n\u003cp\u003e3 months at 2-8 °C.*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3236D\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000E\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eWash Buffer\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of concentrated solution (10x)\u003cem\u003e.\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e6 ml of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003c\/p\u003e\n\u003cp\u003eThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Product\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\"\u003e Human IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-4-elisa-1-step-and-1hr\/\"\u003eHuman IL-4 ELISA (TBS3221)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3236--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3236_human-insulin-elia-V01_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3236_Human-Insulin_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986380382287,"sku":"TBS3236","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3236_insulin.jpg?v=1782809956","url":"https:\/\/www.danyel.co.il\/products\/human-insulin-elisa-1-step-and-1hr-tbs3236","provider":"danyel biotech","version":"1.0","type":"link"}