{"product_id":"human-il-4-elisa-1-step-and-1hr-tbs3221","title":"Human IL-4 ELISA; 1 step and 1hr","description":"\u003cp\u003eInterleukin-4 (IL4), is a secreted protein that belongs to the IL-4 \/ IL-13 family. IL-4 enhances both secretion and cell surface expression of IgE and IgG1. It regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes.\u003c\/p\u003e\n\u003cp\u003eThe Fast Human IL-4 ELISA is a solid phase ELISA designed to measure human IL-4 levels in cell culture supernatants, serum, and plasma.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNovel Proprietary ELISA Approach\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e Only 1 hour,  not 5-6 hrs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e Sample and Detection combined into one step.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEasy:\u003c\/strong\u003e All procedures are performed at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The low detection limit is 8.0pg\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe levels of human IL-4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-4 protein.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed as Fig. 3.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-4 is typically 8.0 pg\/ml. The Intra-CV is 4.8% the Inter-CV is 8.6%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-4. No cross reactivity: Human IL-4 Rα, Mouse IL-4.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-5997 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/simple-assay-procedure3221.png\" alt=\"\" width=\"624\" height=\"215\" data-lazy-sizes=\"auto, (max-width: 624px) 100vw, 624px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11207 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3221-2.jpg\" alt=\"\" width=\"689\" height=\"502\" data-lazy-sizes=\"auto, (max-width: 689px) 100vw, 689px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-5998 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/standard-data3221.png\" alt=\"\" width=\"316\" height=\"266\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 458px\" border=\"1px solid black\" width=\"1265\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-4\u003cbr\u003e\nMicroplate\u003c\/td\u003e\n\u003ctd\u003eTBS3232A\u003c\/td\u003e\n\u003ctd\u003e96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-4.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-4\u003cbr\u003e\nStandard\u003c\/td\u003e\n\u003ctd\u003eTBS3232B\u003c\/td\u003e\n\u003ctd\u003e96-well microplate (12 strips of 8 wells) coated with a\u003cbr\u003e\nCapture Antibody specific for human IL-4.\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3232C\u003c\/td\u003e\n\u003ctd\u003e2.2 ml of human IL-4 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3232D\u003c\/td\u003e\n\u003ctd\u003e2.2 ml of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3232E\u003c\/td\u003e\n\u003ctd\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Product\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-8-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-8 ELISA (TBS3225)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3221--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3221_human_IL4_elisa_08_2020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3221_human_IL4_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986378809423,"sku":"TBS3221","price":1913.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3221_IL4.jpg?v=1782809919","url":"https:\/\/www.danyel.co.il\/products\/human-il-4-elisa-1-step-and-1hr-tbs3221","provider":"danyel biotech","version":"1.0","type":"link"}