{"product_id":"human-il-17a-elisa-1-step-and-1hr-tbs3228","title":"Human IL-17A ELISA; 1 step and 1hr","description":"\u003cp\u003eThe Fast Human IL-17A ELISA is a solid phase ELISA designed to measure human IL-17A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000pg\/mL. The levels of human IL-17A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-17A protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-17A is typically 6.5 pg\/ml. The Intra-CV is 5.39%, and the Inter-CV is \u0026lt; 12%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-17A.\u003cbr\u003e\nNo-cross reactivity: Human INF-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F; Mouse IL-17.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-6189 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2018\/03\/sample-assay.png\" alt=\"\" width=\"657\" height=\"130\" data-lazy-sizes=\"(max-width: 657px) 100vw, 657px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3228--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11215 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3228-2.jpg\" alt=\"\" width=\"857\" height=\"542\" data-lazy-sizes=\"auto, (max-width: 857px) 100vw, 857px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11216 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3228-3.jpg\" alt=\"\" width=\"363\" height=\"275\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1240\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-17A\u003cbr\u003e\nMicroplate\u003c\/td\u003e\n\u003ctd\u003eTBS3228A\u003c\/td\u003e\n\u003ctd\u003e96-well microplate (12 strips of 8 wells) coated with a\u003cbr\u003e\nCapture Antibody specific for human IL-17A.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch\u003cbr\u003e\ncontaining the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-17A\u003cbr\u003e\nStandard\u003c\/td\u003e\n\u003ctd\u003eTBS3228B\u003c\/td\u003e\n\u003ctd\u003e60 μL of Recombinant human L-17A protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost\u003cbr\u003e\nfreezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3228C\u003c\/td\u003e\n\u003ctd\u003e2.2 mL of human IL-17A antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3228D\u003c\/td\u003e\n\u003ctd\u003e120 μL of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3228E\u003c\/td\u003e\n\u003ctd\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use an expired kit for assay.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Product\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-4-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-4 ELISA (TBS3221)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3228--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3228_human_IL17A_elisa_11_2020-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3228_human_IL17A_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986379694159,"sku":"TBS3228","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3228_IL-17A.jpg?v=1782809936","url":"https:\/\/www.danyel.co.il\/products\/human-il-17a-elisa-1-step-and-1hr-tbs3228","provider":"danyel biotech","version":"1.0","type":"link"}