{"product_id":"human-il-12-il-23-p40-elisa-1-step-and-1hr-tbs3269","title":"Human IL-12\/IL-23 p40 ELISA; 1 step and 1hr","description":"\u003cp\u003eInterleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTYPICAL DATA\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSENSITIVITY\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-12\/IL-23 p40 is typically 5 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-CV is \u0026lt; 10%, the Inter-CV is \u0026lt; 12%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSPECIFICITY\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-12\/IL-23 P40.\u003c\/p\u003e\n\u003cp\u003eNo-cross reactivity:\u003c\/p\u003e\n\u003cp\u003eRecombinant mouse IL-12; Recombinant mouse IL-12\/IL-23 p40.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSIMPLE PROCEDURES\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-9217 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204-3-1024x225.png\" alt=\"\" width=\"633\" height=\"139\" data-lazy-sizes=\"auto, (max-width: 633px) 100vw, 633px\" data-was-processed=\"true\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSIMPLE STANDARD PREPARATION\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-9220 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3269-3-1024x597.png\" alt=\"\" width=\"588\" height=\"343\" data-lazy-sizes=\"auto, (max-width: 588px) 100vw, 588px\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eSTANDARD CURVE\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9167 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/std-curve-1.png\" alt=\"\" width=\"351\" height=\"259\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKIT CONTENT AND STORAGE CONDITIONS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"676\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"66\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"274\"\u003e\n\u003cstrong\u003eSTORAGE OF \u003c\/strong\u003e\u003cstrong\u003eOPENED\/ \u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Microplate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269A\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12\/IL-23 p40.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Standard\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269B\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e30 μL of Recombinant human IL-12\/IL-23 p40 protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269C\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e2.1 mL of human IL-12\/IL-23 p40 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"274\"\u003e \n\u003cp\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269D\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269E\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRELATIVE PRODUCTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-4-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-4 ELISA (TBS3221)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-8-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-8 ELISA (TBS3225)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3269--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3269_human_IL12-23-p70_elisa_datasheet_022.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3269_Human_IL-12_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986387198031,"sku":"TBS3269","price":2097.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3269_hp40.jpg?v=1782810267","url":"https:\/\/www.danyel.co.il\/products\/human-il-12-il-23-p40-elisa-1-step-and-1hr-tbs3269","provider":"danyel biotech","version":"1.0","type":"link"}