{"product_id":"human-cell-free-protein-expression-system-3281","title":"Human Cell-Free Protein Expression System","description":"\u003cp\u003eThe Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg\/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThey allow quick analyses of protein functions or features.\u003c\/li\u003e\n\u003cli\u003eThey are amenable to high-throughput studies.\u003c\/li\u003e\n\u003cli\u003eProteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpression of toxic proteins that are lethal for in vivo expression systems\u003cbr\u003eQuick analysis of protein functionality\u003cbr\u003eRapid analysis of protein mutations or truncations\u003cbr\u003eExpression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli\u003cbr\u003eHigh-throughp\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3281\/3281.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3654\/SD3654-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3281\/3281_e.v1807Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pT7-IRES_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epT7-IRES Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45985998274639,"sku":"3281","price":2279.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3281-Human-Cell-Free-Protein-Expression-System-product-600.jpg?v=1782801061","url":"https:\/\/www.danyel.co.il\/products\/human-cell-free-protein-expression-system-3281","provider":"danyel biotech","version":"1.0","type":"link"}