{"product_id":"hitrap-protein-l-antibody-fragment-purification-columns-17547855","title":"HiTrap™ protein L antibody fragment purification columns","description":"\u003cp\u003eWant to learn about our latest protein L resin? Check out HiTrap™ column with MabSelect™ VL resin , with higher binding capacity and more robust alkaline stability than Capto™ L resins. HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSuitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).\u003c\/li\u003e\n\u003cli\u003eEnables a platform approach to antibody fragment purification.\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.\u003c\/li\u003e\n\u003cli\u003eConvenient for process development, as well as small-scale purification.\u003c\/li\u003e\n\u003cli\u003eHiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProtein L chromatography column\u003c\/p\u003e\n\n\u003cp\u003eHiTrap™ Protein L column is packed with \u003ca href=\"\/shop\/chromatography\/resins\/affinity-antibody\/capto-l-antibody-fragment-purification-resin-p-00629\"\u003eCapto™ L resin\u003c\/a\u003e. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.\u003c\/p\u003e\n\n\u003cp\u003eThe high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody, scFv, and Fab purification.\u003c\/p\u003e\n\n\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\n\n\u003cp\u003eHiTrap™ Protein L is used in a variety of research applications, including:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003eCharacterization of a \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3856173\/\"\u003ehuman colorectal cancer MDR1\/P-gp Fab\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003ePurification optimization of an \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26297993\/\"\u003escFv against type 1 insulin-like growth factor receptor (IGF-1R)\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eOther Cytiva Capto™ L chromatography columns and formats\u003c\/p\u003e\n\n\u003cp\u003eCapto™ L resin is also available in the following formats:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003e\u003ca href=\"\/shop\/chromatography\/resins\/affinity-antibody\/capto-l-antibody-fragment-purification-resin-p-00629\"\u003eBulk Capto™ L resin\u003c\/a\u003e\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/affinity-antibody\/hiscreen-capto-l-p-05667\"\u003eHiScreen Capto™ L\u003c\/a\u003e prepacked column, for method and process development\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/affinity-antibody\/predictor-capto-l-p-06165\"\u003ePreDictor Capto™ L\u003c\/a\u003e 96-well plate for high-throughput screening or process development.\u003cbr\u003e\n\t \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16102-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29007877.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 1 mL","offer_id":45982360469583,"sku":"29048665","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 × 5 mL","offer_id":45982360502351,"sku":"17547815","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 1 mL","offer_id":45982360535119,"sku":"17547851","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 5 mL","offer_id":45982360567887,"sku":"17547855","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-3775-original-png_28799f24-26a7-4268-9fd9-6c346e7bdfa4.png?v=1785960496","url":"https:\/\/www.danyel.co.il\/products\/hitrap-protein-l-antibody-fragment-purification-columns-17547855","provider":"danyel biotech","version":"1.0","type":"link"}