{"product_id":"hitrap-protein-g-hp-antibody-purification-columns-29048581","title":"HiTrap™ Protein G HP antibody purification columns","description":"\u003cp\u003eHiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFirst choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.\u003c\/li\u003e\n\u003cli\u003eThe small bead size (34 μm) ensures narrow elution peaks containing concentrated material.\u003c\/li\u003e\n\u003cli\u003eHigh protein recovery due to high binding capacity of 25 mg IgG\/mL resin.\u003c\/li\u003e\n\u003cli\u003eBroader selectivity compared with protein A ligand.\u003c\/li\u003e\n\u003cli\u003eConvenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProtein G column for general-purpose antibody purification\u003c\/p\u003e\u003cp\u003eHiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance resin. This  protein G affinity resin is a good first choice for general-purpose capture of antibodies at laboratory scale, because it binds to a broader range of IgG from eukaryotic species and also binds to more subclasses of IgG than protein A-based resins do.\u003c\/p\u003e\u003cp\u003eUse these columns for IgG purification from cow, sheep, horse, rat, mouse, and human sources, including ascites, serum, and cell culture supernatants.\u003c\/p\u003e\u003cp\u003eHiTrap Protein G HP columns deliver high-performance, high-resolution purifications with a binding capacity of 25 mg IgG\/mL resin and fast kinetics. Columns are stable over a wide pH range – operational pH 3 to 9, and pH 2 to 10 for cleaning.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eProtein G HiTrap columns are relied upon for a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePurification of primary antibody from rabbit for research on \u003ca href=\"https:\/\/onlinelibrary.wiley.com\/action\/cookieAbsent\"\u003e human lung cells \u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003emAb purification from ascites for research on the \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/9396465\/\"\u003eprotein C anticoagulant pathway\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of IgG \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11238669\"\u003eantifilaggrin autoantibodies\u003c\/a\u003e from human sera\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eOther formats of Protein G Sepharose HP resin\u003c\/p\u003e\u003cp\u003eProtein G Sepharose High Performance resin is also available in the following formats: \n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eProtein G HP SpinTrap spin columns, for small sample purification using a microcentrifuge\u003c\/li\u003e\n\u003cli\u003eProtein G HP MultiTrap 96-well plates, for high-throughput screening and small-scale purification using centrifuge or vacuum equipment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13703-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11420-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 1 mL","offer_id":45982108975183,"sku":"29048581","price":1193.0,"currency_code":"ILS","in_stock":true},{"title":"1 × 5 mL","offer_id":45982109007951,"sku":"17040501","price":3782.0,"currency_code":"ILS","in_stock":true},{"title":"2 × 1 mL","offer_id":45982109040719,"sku":"17040403","price":1610.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 1 mL","offer_id":45982109073487,"sku":"17040401","price":4332.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 5 mL","offer_id":45982109106255,"sku":"17040503","price":13380.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7778-original-png_e6ae4c7e-8a74-4231-83c8-78e674890072.png?v=1785958402","url":"https:\/\/www.danyel.co.il\/products\/hitrap-protein-g-hp-antibody-purification-columns-29048581","provider":"danyel biotech","version":"1.0","type":"link"}