{"product_id":"hitrap-nhs-activated-hp-affinity-columns-17071701","title":"HiTrap™ NHS-Activated HP affinity columns","description":"\u003cp\u003eHiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEasy coupling of any ligand containing primary amine groups.\u003c\/li\u003e\n\u003cli\u003ePacked with NHS-Activated Sepharose High Performance resin.\u003c\/li\u003e\n\u003cli\u003eConvenient prepacked columns can be used with a syringe, pump, or chromatographic system, such as ÄKTA systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNHS activated resin for amine coupling\u003c\/p\u003e\u003cp\u003eThis NHS-activated resin can be used with any primary amine-containing ligand. The resin reacts with primary amine groups to form stable amide linkages that covalently immobilize antibodies or other proteins for affinity purification. The resin enables simple coupling, carried out at close to neutral pH.\u003c\/p\u003e\u003cp\u003eThe NHS-activated Sepharose High Performance (HP) resin in these columns is delivered in 100% isopropanol to protect the active groups. It contains ~ 10 μmol NHS groups\/mL resin.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eHiTrap NHS-activated Sepharose HP columns are used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23308125\/\"\u003eCharacterizing immunoglobulins\u003c\/a\u003e using anti-Fab affinity chromatography\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3807418\/\"\u003eStudying systemic transduction\u003c\/a\u003e of recombinant adeno-associated virus vectors in mice \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11509-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11233-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 5 mL","offer_id":45982107926607,"sku":"17071701","price":1109.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 1 mL","offer_id":45982107959375,"sku":"17071601","price":1260.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7770-original-png_93318650-73ae-4c68-b53d-d25768698de3.png?v=1785958376","url":"https:\/\/www.danyel.co.il\/products\/hitrap-nhs-activated-hp-affinity-columns-17071701","provider":"danyel biotech","version":"1.0","type":"link"}