{"product_id":"hitrap-fibro-and-hiscreen-fibro-prisma-protein-a-chromatography-17549855","title":"HiTrap Fibro™ and HiScreen Fibro™ PrismA protein A chromatography","description":"\u003cp\u003eHiTrap Fibro™ and HiScreen Fibro™ PrismA are protein A fiber chromatography units for rapid cycling chromatography purification of mAbs for research and process development applications. Ultrafast purification enables cycle times under 5 minutes compared to hours for resins. HiTrap Fibro™ PrismA is primarily intended for reserach use and HiScreen Fibro™ PrismA is primarily intended for early process development.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePurify mAbs and Fc-containing fragments using rapid cycling chromatogrpaphy. Cycle times are less than 5 minutes compared with hours for chromatography resins.\u003c\/li\u003e\n\u003cli\u003eIncrease throughput up to 20-fold over that for resin-based chromatography and perform a full life time study in one day. This cuts weeks from lead times in process development.\u003c\/li\u003e\n\u003cli\u003eAchieve high-throughput purification of up to 500 mAbs per week for cell line or candidate screening.\u003c\/li\u003e\n\u003cli\u003eRun Fibro PrismA units on an ÄKTA™ chromatography system for real-time UV, pH and conductivity detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe HiTrap Fibro™ and HiScreen Fibro™ PrismA adsorbents have a protein A cellulose fiber matrix with an open pore structure where mass transfer is governed by convective flow. This structure allows high mAb binding capacities at very short residence times by using rapid cycling chromatography. This results in cycle times of minutes instead of the hours needed for resin-based chromatography. Dynamic binding capacity is ~ 30 mg IgG\/mL matrix. The Fibro technology platform is designed to scale up to manufacturing scale. The units will range from research scale with a capacity of approximately 12 mg per purification cycle (0.4 mL units) to GMP compatible units that can process up to 2000 L bioreactor harvest or 10 kg mAb in one working day by cycling multiple times.\u003c\/p\u003e\n\n\u003cp\u003eApplications\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/Intensified-Chromatography-Strategies\"\u003eIntensify protein A chromatography\u003c\/a\u003e.\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/chromatography-productivity-with-fiber-adsorbents\"\u003eOvercome chromatography challenges\u003c\/a\u003e.\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/fast-two-step-mAb-purification\"\u003eIncrease throughput with this automated two-step process\u003c\/a\u003e.\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/fiber-based-protein-a-chromatography-for-htpd\"\u003eCut lead times dramatically using high-throughput process development\u003c\/a\u003e.\u003c\/li\u003e\n\t\u003cli\u003e\n\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/Fibro-PrismA-high-throughput-purification\"\u003eCustomer Case study: High throughput mAb purification\u003c\/a\u003e.\u003c\/li\u003e\n\t\u003cli\u003e\u003ca href=\"\/solutions\/bioprocessing\/knowledge-center\/Top-questions-about-Fibro\"\u003e14 questions of Fibro chromatography.\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003eGetting started\u003c\/p\u003e\n\n\u003cp\u003eUsing resin-based columns? Fibro PrismA units connect to ÄKTA™ systems in the same way. And you can use the same buffers.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca href=\"\/education\/online-learning\/courses\"\u003eFree E-learning: Getting started with Fibro\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-33339-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-33174-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 0.4 mL","offer_id":45982416896079,"sku":"17549855","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 × 3.75 mL","offer_id":45982416928847,"sku":"17549816","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"4 × 0.4 mL","offer_id":45982416961615,"sku":"17549856","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-44420-original-png_24be940a-054b-459c-92a2-70a45867eb86.png?v=1785960876","url":"https:\/\/www.danyel.co.il\/products\/hitrap-fibro-and-hiscreen-fibro-prisma-protein-a-chromatography-17549855","provider":"danyel biotech","version":"1.0","type":"link"}