{"product_id":"histrap-ff-crude-histidine-tagged-protein-purification-columns-17528601","title":"HisTrap™ FF Crude histidine-tagged protein purification columns","description":"\u003cp\u003eHisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh binding capacity – binds up to 40 mg his-tag protein\/mL resin.\u003c\/li\u003e\n\u003cli\u003eExcellent flow properties – great performance even at high flow rates.\u003c\/li\u003e\n\u003cli\u003eOptimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins.\u003c\/li\u003e\n\u003cli\u003eHiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHis-tag protein purification from unclarified lysates\u003c\/p\u003e\n\n\u003cp\u003eHisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.\u003c\/p\u003e\n\n\u003cp\u003eIf you plan to load unclarified samples, it is important to ensure that lysis is as complete as possible. We recommend adding lysozyme and DNase I, followed by mechanical lysis (e.g., by sonication) for a longer duration than typically performed.\u003c\/p\u003e\n\n\u003cp\u003eTips and tricks for using these IMAC columns\u003c\/p\u003e\n\n\u003cp\u003eInterested in maximizing the performance of your column? \u003ca href=\"https:\/\/share.vidyard.com\/watch\/DXyQHt9Aq2SQAvRmrr3pgq?\"\u003e Watch Cytiva's HisTrap FF crude column video\u003c\/a\u003e to get hints and recommendations.\u003c\/p\u003e\n\n\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\n\n\u003cp\u003eThese columns are used in a variety of applications, including:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003ePurification of a his-tagged ferritin protein \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22240114\/\"\u003efrom unclarified \u003ci\u003eE. coli\u003c\/i\u003e lysates\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003ePurification of unphosphorylated \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19864428\/\"\u003ehuman ribosomal protein\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003eRecombinant protein purification to \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18625781\/\"\u003estudy the mechanism of action of antibiotic NXL101\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eOther Cytiva products for purifying his-tagged proteins\u003c\/p\u003e\n\n\u003cp\u003eCytiva provides a large variety of Life Sciences solutions for IMAC protein purification. For routine his-tagged protein purification or when high resolution is a priority, consider HisTrap HP columns.\u003c\/p\u003e\n\n\u003cp\u003eNot sure which product to use? Let Cytiva help you find the right IMAC purification resin or column for your needs for your needs.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13646-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13615-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 1 mL","offer_id":45982408474703,"sku":"29048631","price":298.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 1 mL","offer_id":45982408507471,"sku":"11000458","price":1136.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 5 mL","offer_id":45982408540239,"sku":"17528601","price":4087.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-3791-original-png_02844280-c09f-4dcb-85da-82c5f9e5c87a.png?v=1785960776","url":"https:\/\/www.danyel.co.il\/products\/histrap-ff-crude-histidine-tagged-protein-purification-columns-17528601","provider":"danyel biotech","version":"1.0","type":"link"}