{"product_id":"histrap-ff-columns-for-high-capacity-protein-purification-17525501","title":"HisTrap™ FF columns for high-capacity protein purification","description":"\u003cp\u003ePrepacked Ni2+ Sepharose columns for histidine-tagged protein purification\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh binding capacity, approx. 40 mg\/mL resin.\u003c\/li\u003e\n\u003cli\u003eNegligible leakage of Ni2+.\u003c\/li\u003e\n\u003cli\u003ePrepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.\u003c\/li\u003e\n\u003cli\u003eCompatible with a wide range of reducing agents, detergents, denaturants, and other additives.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNi Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni\u003csup\u003e2+\u003c\/sup\u003e ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni\u003csup\u003e2+\u003c\/sup\u003e ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications\u003c\/p\u003e\u003cp\u003eThe high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13253-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13165-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"5 × 1 mL","offer_id":45982409785423,"sku":"17531901","price":818.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 5 mL","offer_id":45982409818191,"sku":"17525501","price":2819.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7809-original-png_c0b58d89-37ae-4005-8eff-a43342605028.png?v=1785960789","url":"https:\/\/www.danyel.co.il\/products\/histrap-ff-columns-for-high-capacity-protein-purification-17525501","provider":"danyel biotech","version":"1.0","type":"link"}